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His-PLK1 full length purified from Sf9 cells.
This antibody is available as an azide-free product (see ab178666).
Our Abpromise guarantee covers the use of ab17057 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 66 kDa (predicted molecular weight: 68 kDa).|
|ICC/IF||1/200. PubMed: 19033445|
|Flow Cyt||Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
Immunofluoresence using ab17057 and either HeLaS3, NIH 3T3 or U2OS cells.
Western blot using ab17057.
Lane1: recombinant Plk1
Lane 2: U2OS cell extract
Lane 3: HeLaS3 cell extract
10% SDS-PAGE gel.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"