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Recombinant full length protein (Xenopus laevis).
Our Abpromise guarantee covers the use of ab12210 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent dilution.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 68 kDa).|
Western blot using ab12210.
Lane 1: HeLa extract
Lane 2: mouse Myeloid cell extract
Lane 3: Mitotic CSF Xenopus egg extract
Lane 4: Interphase Xenopus egg extract M
olecular weight markers 120, 98, 68, 57, 43, 32, 18, 12 kD.
Note that the phosphorylated form of Polo kinase from the Xenopus mitotic extract migrates slower than the unphosphorylated form present in the interphase extract.
Julian Gannon, CRUK
ab12210 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"