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Recombinant full length protein (Xenopus laevis).
Our Abpromise guarantee covers the use of ab12211 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent dilution.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 68 kDa).|
Lane 1: Mitotic CSF Xenopus egg extract
Lane 2: Interphase Xenopus egg extract
Lane 3: HeLa cell extract
Note that the phosphorylated form of Plx from the Xenopus mitotic extract migrates slower than the unphosphorylated form present in the interphase extract.
Julian Gannon, CRUK
ab12211 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"