Anti-PLK1 antibody [AZ34] (ab12211)

Overview

  • Product nameAnti-PLK1 antibody [AZ34]See all PLK1 primary antibodies ...
  • Description
    Mouse monoclonal [AZ34] to PLK1
  • SpecificityThe cross-reactivity of this antibody regards the various isoforms of PLK is not known.
  • Tested applicationsELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human, Xenopus laevis
  • Immunogen

    Recombinant full length protein (Xenopus laevis).

  • Positive control
    • Hela cells

Properties

Applications

Our Abpromise guarantee covers the use of ab12211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 68 kDa).
  • Application notesIs unsuitable for ICC/IF.
  • Target

    • FunctionSerine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS.
    • Tissue specificityPlacenta and colon.
    • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
      Contains 2 POLO box domains.
      Contains 1 protein kinase domain.
    • Developmental stageAccumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.
    • Post-translational
      modifications
      Catalytic activity is enhanced by phosphorylation of Thr-210. Phosphorylation at Thr-210 is first detected on centrosomes in the G2 phase of the cell cycle, peaks in prometaphase and gradually disappears from centrosomes during anaphase.
      Autophosphorylation and phosphorylation of Ser-137 may not be significant for the activation of PLK1 during mitosis, but may enhance catalytic activity during recovery after DNA damage checkpoint.
      Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) in anaphase and following DNA damage, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Ubiquitination and subsequent degradation prevents entry into mitosis and is essential to maintain an efficient G2 DNA damage checkpoint.
    • Cellular localizationNucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > centrosome. During early stages of mitosis, the phosphorylated form is detected on centrosomes and kinetochores. Localizes to the outer kinetochore. Presence of SGOL1 and interaction with the phosphorylated form of BUB1 is required for the kinetochore localization.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cell cycle regulated protein kinase antibody
      • PLK 1 antibody
      • PLK antibody
      • PLK-1 antibody
      • plk1 antibody
      • PLK1_HUMAN antibody
      • polio like kinase antibody
      • Polo like kinase 1 antibody
      • Polo-like kinase 1 antibody
      • Serine/threonine protein kinase antibody
      • Serine/threonine protein kinase 13 antibody
      • Serine/threonine protein kinase PLK1 antibody
      • Serine/threonine-protein kinase 13 antibody
      • Serine/threonine-protein kinase PLK1 antibody
      • STPK 13 antibody
      • STPK13 antibody
      see all

    Anti-PLK1 antibody [AZ34] images



    • Predicted band size : 68 kDa


      Western blot using ab12211.

      Lane 1: Mitotic CSF Xenopus egg extract
      Lane 2: Interphase Xenopus egg extract
      Lane 3: HeLa cell extract
      Note that the phosphorylated form of Plx from the Xenopus mitotic extract migrates slower than the unphosphorylated form present in the interphase extract.

      Julian Gannon, CRUK

    • Anti-PLK1 antibody [AZ34] (ab12211) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

      Secondary
      Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Predicted band size : 68 kDa
      Observed band size : 57 kDa (why is the actual band size different from the predicted?)

    References for Anti-PLK1 antibody [AZ34] (ab12211)

    ab12211 has not yet been referenced specifically in any publications.

    Product Wall

    Many thanks for the image, it is very useful. I would recommend incubating the membrane in the antibody overnight at 4C in TBST and also trying more concentrated antibody (such as 2-5ug/ml), if you still experience problems with those conditions ple...

    Read More

    I'm sorry to hear you are having no signal with ab12211 in WB. Could you please tell me the dilution and incubation time you have tested and send me an image of the blot? I look forward to hearing from you, Thank you in advance,

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"