Anti-PLK1 (phospho S137) antibody (ab21738)

Overview

  • Product nameAnti-PLK1 (phospho S137) antibodySee all PLK1 primary antibodies ...
  • Description
    Rabbit polyclonal to PLK1 (phospho S137)
  • Tested applicationsIP, ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Xenopus laevis
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human PLK1, phosphorylated at S137.

    (Peptide available as ab25712.)

  • Positive control
    • This antibody gave a positive signal in Human normal colon FFPE section. IF/ICC: MCF7 cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab21738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.

AP-791911

IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Can be blocked with Human PLK1 (phospho S137) peptide (ab25712).

Target

  • FunctionSerine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS.
  • Tissue specificityPlacenta and colon.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
    Contains 2 POLO box domains.
    Contains 1 protein kinase domain.
  • Developmental stageAccumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.
  • Post-translational
    modifications
    Catalytic activity is enhanced by phosphorylation of Thr-210. Phosphorylation at Thr-210 is first detected on centrosomes in the G2 phase of the cell cycle, peaks in prometaphase and gradually disappears from centrosomes during anaphase.
    Autophosphorylation and phosphorylation of Ser-137 may not be significant for the activation of PLK1 during mitosis, but may enhance catalytic activity during recovery after DNA damage checkpoint.
    Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) in anaphase and following DNA damage, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Ubiquitination and subsequent degradation prevents entry into mitosis and is essential to maintain an efficient G2 DNA damage checkpoint.
  • Cellular localizationNucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > centrosome. During early stages of mitosis, the phosphorylated form is detected on centrosomes and kinetochores. Localizes to the outer kinetochore. Presence of SGOL1 and interaction with the phosphorylated form of BUB1 is required for the kinetochore localization.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell cycle regulated protein kinase antibody
    • PLK 1 antibody
    • PLK antibody
    • PLK-1 antibody
    • plk1 antibody
    • PLK1_HUMAN antibody
    • polio like kinase antibody
    • Polo like kinase 1 antibody
    • Polo-like kinase 1 antibody
    • Serine/threonine protein kinase antibody
    • Serine/threonine protein kinase 13 antibody
    • Serine/threonine protein kinase PLK1 antibody
    • Serine/threonine-protein kinase 13 antibody
    • Serine/threonine-protein kinase PLK1 antibody
    • STPK 13 antibody
    • STPK13 antibody
    see all

Anti-PLK1 (phospho S137) antibody images

  • ICC/IF image of ab21738 stained MCF7 cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab21738, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 Goat anti-Rabit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of PLK1 (phospho S137) staining in human normal colon FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab21738, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
  • This antibody gives a positive result in ELISA against the modified peptide ab25712 (orange dotted line) but does not recognise the corresponding non modified peptide ab25713 (yellow dotted line). This indicates that ab21738 specifically recognises PLK1 phosphorylated at S137.     

  • PLK1 (phospho S137) immunoprecipitation from Human HeLa cells using ab21738. 200 µg of cell lysate was incubated with primary antibody (15 µg/mg cell lysate) and matrix (Protein G) and incubated for 2 hour 4°C.

    Lane 1: Control beads
    Lane 2: Anti-PLK1 (phospho S137) IP

    Immunoprecipitation was confirmed by western blot with an HRP-conjugated Mouse monoclonal antibody against pan-PLK1 (1/5000)

    See Abreview

References for Anti-PLK1 (phospho S137) antibody (ab21738)

ab21738 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Colon carcinoma)
Loading amount 50 µg
Specification Colon carcinoma
Gel Running Conditions Non-reduced Denaturing (10)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Eleni Petsalaki

Verified customer

Submitted Dec 03 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (Hela)
Total protein in input 200 µg
Specification Hela
Treatment lane 1: control beads Lane 2: IP with ab21738 antibody
Immuno-precipitation step Protein G
Username

Abcam user community

Verified customer

Submitted Aug 01 2012

DISCOUNT CODE: *** Expiration date: February 19, 2012 This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab21738 in Western blot and include this code in the “Additional Comme...

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Application Western blot
Sample Human Cell lysate - whole cell (U87 cells)
Loading amount 30 µg
Specification U87 cells
Treatment 0.5% DMSO and drug for 1hr
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 28 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"