Anti-PLK1 (phospho T210) antibody [2A3] (ab39068)

Overview

  • Product nameAnti-PLK1 (phospho T210) antibody [2A3]See all PLK1 primary antibodies ...
  • Description
    Mouse monoclonal [2A3] to PLK1 (phospho T210)
  • Specificityab39068 recognises Plk1 (phospho T210).
  • Tested applicationsICC/IF, Flow Cyt, WB more details
  • Species reactivity
    Reacts with: Chicken, Human
  • Immunogen

    Synthetic peptide (Human): Modified peptide

  • Positive control
    • Extracts from overnight nocodazole treated Hela cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab39068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF
Flow Cyt
WB
  • Application notesFlow Cyt: Use at an assay dependent dilution.
    ICC/IF: 1/300.
    WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 68 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionSerine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS.
    • Tissue specificityPlacenta and colon.
    • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
      Contains 2 POLO box domains.
      Contains 1 protein kinase domain.
    • Developmental stageAccumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.
    • Post-translational
      modifications
      Catalytic activity is enhanced by phosphorylation of Thr-210. Phosphorylation at Thr-210 is first detected on centrosomes in the G2 phase of the cell cycle, peaks in prometaphase and gradually disappears from centrosomes during anaphase.
      Autophosphorylation and phosphorylation of Ser-137 may not be significant for the activation of PLK1 during mitosis, but may enhance catalytic activity during recovery after DNA damage checkpoint.
      Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) in anaphase and following DNA damage, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Ubiquitination and subsequent degradation prevents entry into mitosis and is essential to maintain an efficient G2 DNA damage checkpoint.
    • Cellular localizationNucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > centrosome. During early stages of mitosis, the phosphorylated form is detected on centrosomes and kinetochores. Localizes to the outer kinetochore. Presence of SGOL1 and interaction with the phosphorylated form of BUB1 is required for the kinetochore localization.
    • Target information above from: UniProt accession P53350 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • Cell cycle regulated protein kinase antibody
      • PLK 1 antibody
      • PLK antibody
      • PLK-1 antibody
      • plk1 antibody
      • PLK1_HUMAN antibody
      • polio like kinase antibody
      • Polo like kinase 1 antibody
      • Polo-like kinase 1 antibody
      • Serine/threonine protein kinase 13 antibody
      • Serine/threonine protein kinase PLK1 antibody
      • Serine/threonine-protein kinase 13 antibody
      • Serine/threonine-protein kinase antibody
      • Serine/threonine-protein kinase PLK1 antibody
      • STPK 13 antibody
      • STPK13 antibody
      see all

    Anti-PLK1 (phospho T210) antibody [2A3] images

    • All lanes : Anti-PLK1 (phospho T210) antibody [2A3] (ab39068) at 1 µg/ml

      Lane 1 : Extracts from untreated Hela cells
      Lane 2 : Extracts from overnight nocodazole treated Hela cells


      Predicted band size : 68 kDa
      Observed band size : 66 kDa (why is the actual band size different from the predicted?)
    • ab39068 staining chicken B lymphoma cells in prometaphase by ICC/IF. Cells were PFA fixed and permeabilized in 0.15% Triton X-100 prior to blocking in 1% BSA for 1 hour at 4°C. The primary antibody was diluted 1/300 (PBS/1%BSA) and incubated with the sample for 1 hour at 37°C. A Texas Red® conjugated goat polyclonal to mouse, diluted 1/200, was used as the secondary.

      See Abreview

    References for Anti-PLK1 (phospho T210) antibody [2A3] (ab39068)

    This product has been referenced in:
    • Tan EP  et al. O-linked N-acetylglucosamine cycling regulates mitotic spindle organization. J Biol Chem 288:27085-99 (2013). Read more (PubMed: 23946484) »
    • Kasahara K  et al. PI 3-kinase-dependent phosphorylation of Plk1-Ser99 promotes association with 14-3-3? and is required for metaphase-anaphase transition. Nat Commun 4:1882 (2013). Human . Read more (PubMed: 23695676) »

    See all 4 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 50 µg
    Gel Running Conditions Non-reduced Denaturing (10%)
    Sample Human Cell lysate - whole cell (Colon carcinoma)
    Specification Colon carcinoma
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Dr. Eleni Petsalaki

    Verified customer

    Submitted Dec 04 2012

    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa)
    Loading amount 100 µg
    Specification HeLa
    Gel Running Conditions Reduced Denaturing (12% gel)
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
    Username

    Ms. AeLee Jeong

    Verified customer

    Submitted Sep 25 2012

    Thank you for contacting us.
    Your credit note ID is xxx.
    I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoic...

    Read More

    Thank you for your reply and the additional information.
    I can offer you a free of charge replacement with another antibody of your choice, a credit or refund.
    Please let me know what option would be best for you.
    I will also get in touc...

    Read More

    Thank you for contacting us.
    I'm sorry to hear that the antibody does not seem to work as it should.
    Could you please let me know the following information in order to understand the situation better:
    1) What samples (species, type) are ...

    Read More
    Application Western blot
    Sample Human Cell lysate - whole cell (Hela)
    Loading amount 50 µg
    Specification Hela
    Gel Running Conditions Reduced Denaturing (10)
    Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 23 2012

    I am very pleased to hear you would like to accept our offer and test ab39068 in MEFs on Western Blot. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for MEFs on Western Blot ...

    Read More

    Thank you very much for your interest in ab39068.

    To our knowledge, this has not been tested in Western blot on mouse ebryonic fibroblasts (MEFs). Therefore, I can offer a discount off a future purchase if you buy ab39068 now, test it in W...

    Read More

    Thank you for your inquiry.

    While we do not list this as cross reacting with mouse, it is possibnle that it may as the regions are so highly conserved. We generally do not recommend using an antibody created in mouse on mouse tissue because ...

    Read More

    Thank you for contacting Abcam.

    The immunogen used for this product has 100% homology with the mouse protein (http://www.uniprot.org/uniprot/Q07832). This product has not yet been tested in this species however. Because this has been raised ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"