Recombinant Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPNCIR167] to PLK1 (phospho T210)
- Suitable for: WB, IHC-P, Dot blot
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-PLK1 (phospho T210) antibody [EPNCIR167]
See all PLK1 primary antibodies -
Description
Rabbit monoclonal [EPNCIR167] to PLK1 (phospho T210) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Dot blotmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, NIH/3T3, Mouse testis, Mouse colon, Hela treated with thymidine and nocodazole, HeLa treated with nocodazole, HeLa treated with calyculin A cell lysates. Dot: PLK1 (pT210) phospho peptide. IHC-P: Human colon, gastroic carcinoma, thyroid gland carcinoma, cervical carcinoma and placenta tissues.
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General notes
This antibody was developed as part of a collaboration between the National Cancer Institute's Center for Cancer Research and the lab of Kyung Lee. View antibodies from NCI Center for Cancer Research Collaboration.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPNCIR167 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155095 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 68 kDa.
For unpurified use at 1/1000 - 1/10000. |
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IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250. |
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Dot blot |
Use at an assay dependent concentration.
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Notes |
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WB
1/1000. Predicted molecular weight: 68 kDa. For unpurified use at 1/1000 - 1/10000. |
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250. |
Dot blot
Use at an assay dependent concentration. |
Target
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Function
Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS. -
Tissue specificity
Placenta and colon. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
Contains 2 POLO box domains.
Contains 1 protein kinase domain. -
Developmental stage
Accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase. -
Post-translational
modificationsCatalytic activity is enhanced by phosphorylation of Thr-210. Phosphorylation at Thr-210 is first detected on centrosomes in the G2 phase of the cell cycle, peaks in prometaphase and gradually disappears from centrosomes during anaphase.
Autophosphorylation and phosphorylation of Ser-137 may not be significant for the activation of PLK1 during mitosis, but may enhance catalytic activity during recovery after DNA damage checkpoint.
Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) in anaphase and following DNA damage, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Ubiquitination and subsequent degradation prevents entry into mitosis and is essential to maintain an efficient G2 DNA damage checkpoint. -
Cellular localization
Nucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > centrosome. During early stages of mitosis, the phosphorylated form is detected on centrosomes and kinetochores. Localizes to the outer kinetochore. Presence of SGOL1 and interaction with the phosphorylated form of BUB1 is required for the kinetochore localization. - Information by UniProt
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Database links
- Entrez Gene: 5347 Human
- Entrez Gene: 18817 Mouse
- Omim: 602098 Human
- SwissProt: P53350 Human
- SwissProt: Q07832 Mouse
- Unigene: 592049 Human
- Unigene: 16525 Mouse
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Alternative names
- Cell cycle regulated protein kinase antibody
- PLK 1 antibody
- PLK antibody
see all
Images
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All lanes : Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095) at 1/1000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) (RIPA) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) (RIPA) whole cell lysate
Lane 3 : Mouse testis (RIPA) lysate
Lane 4 : Mouse colon (RIPA) lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Exposure Time: Lane 1-2: 40 seconds, Lane 3: 3 seconds and Lane 4: 180 seconds.
ab181602 was used as a loading control.
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All lanes : Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095) at 1/1000 dilution
Lane 1 : Hela (Human cervix adenocarcinoma epithelial cell) whole cell lysates with NFDM/TBST
Lane 2 : Hela (Human cervix adenocarcinoma epithelial cell) treated with thymidine (2mM, 16 h) then with nocodazole (10nM, 24h). Whole cell lysates with NFDM/TBST
Lane 3 : Hela (Human cervix adenocarcinoma epithelial cell) treated with thymidine (2mM, 16 h) then with nocodazole (10nM, 24h). Whole cell lysates.Then the membrane was incubated with phosphatase. with NFDM/TBST
Lysates/proteins at 15 µg per lane.
Blocking peptides at 5 % per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDa
Exposure time: 10 secondsantibody used for ab155095 is purified batch
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Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095) Dot Blot. Primary ab dilution 1:1000, Secondary ab description and code (ab id)Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051), Secondary ab dilution 1:100,000. Blocking buffer and concentration 5% NFDM/TBST, Diluting buffer and concentration 5% NFDM /TBST. Lane 1:PLK1 (pT210) phospho peptide, Lane 2: PLK1 non-phospho peptide, Exposure time 10 seconds. Note: antibody used for ab155095 is purified batch.
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ab155095 staining PLK1 (phospho T210) in Human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/500). An undiluted HRP-conjugated anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.
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All lanes : Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HeLa cell lysate post treatment with Nocodazole
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution
Predicted band size: 68 kDa -
All lanes : Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095) at 1/1000 dilution (unpurified)
Lane 1 : HeLa cell lysate, untreated
Lane 2 : HeLa cell lysate, treated with calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 68 kDa -
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PLK1 with ab155095, unpurified, at 1/100 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human gastroic carcinoma tissue labeling PLK1 with ab155095, unpurified, at 1/100 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab155095, unpurified, showing +ve staining.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue using ab155095, unpurified, showing +ve staining.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human placenta tissue using ab155095, unpurified, showing +ve staining.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab155095 has been referenced in 14 publications.
- Zhang Z et al. PLK1 Mitigates Intervertebral Disc Degeneration by Delaying Senescence of Nucleus Pulposus Cells. Front Cell Dev Biol 10:819262 (2022). PubMed: 35372354
- Kamranvar SA et al. Integrin-Mediated Adhesion Promotes Centrosome Separation in Early Mitosis. Cells 11:N/A (2022). PubMed: 35456039
- Zhang C et al. Icaritin inhibits PLK1 to activate DNA damage response in NK/T cell lymphoma and increases sensitivity to GELOX regime. Mol Ther Oncolytics 25:288-304 (2022). PubMed: 35663228
- Sun EC et al. Clinicopathological Significance of AKT1 and PLK1 Expression in Oral Squamous Cell Carcinoma. Dis Markers 2022:7300593 (2022). PubMed: 35756492
- Qiu Q et al. A prognosis model for clear cell renal cell carcinoma based on four necroptosis-related genes. Front Med (Lausanne) 9:942991 (2022). PubMed: 36016998