Anti-PLVAP antibody [174/2] (ab81719)

Overview

• Product nameAnti-PLVAP antibody [174/2]See all PLVAP primary antibodies ...
• Description
  Mouse monoclonal [174/2] to PLVAP
• Tested applicationsWB, IHC-Fr, Flow Cyt, ICC/IF more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Isolated hilar vessels from human lymph nodes

• Positive controlHuman tonsil cells.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 0.1% BSA, PBS
• Concentration information loading...
• PurityProtein G purified
• Purification notesab81719 is 0.2µM filtered.
• Clonality Monoclonal
• Clone number174/2
• IsotypeIgG1
• Research Areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Cell Adhesion
  • Cell Adhesion Molecules
  • Endothelial

  • Tags & Cell Markers
  • Subcellular Markers
  • Organelles
  • Caveolae and Clathrin

Applications

Target

• FunctionInvolved in the formation of stomatal and fenestral diaphragms of caveolae. May function in microvascular permeability.
• Tissue specificityExpressed in lung, kidney, heart, aorta, placenta, muscle, pituitary gland, adrenals, mammary gland, bladder, lymph node, bone marrow, trachea, digestive tract, liver and tumor-associated endothelium.
• Cellular localizationCell membrane. Membrane > caveola. Cytoplasm > perinuclear region. Membrane-associated protein of caveolae. Found in fenestral and stomatal diaphragms in fenestrated endothelia and transendothelial channels. Also colocalized with CAV1 in perinuclear region.

Target information above from: UniProt accession Q9BX97 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 83483 Human
  • Entrez Gene: 83483 Human
  • Omim: 607647 Human
  • SwissProt: Q9BX97 Human
  • SwissProt: Q9BX97 Human
  • Unigene: 107125 Human

• Alternative names
  FELS antibodyFenestrated endothelial linked structure protein antibodyFenestrated endothelial-linked structure protein antibody

  gp68 antibodyHypothetical protein DKFZp667O074 antibodyMeca 32 antigen antibodyMECA32 antigen antibodyPanendothelial cell antigen antibodyPlasmalemma vesicle associated protein antibodyPlasmalemma vesicle protein 1 antibodyPlasmalemma vesicle-associated protein antibodyPlvap antibodyPLVAP_HUMAN antibodyPV 1 antibodyPV-1 antibodyPV1 antibodyPV1 protein antibody

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Anti-PLVAP antibody [174/2] images

• 

  Immunocytochemistry/ Immunofluorescence - PLVAP antibody [174/2] (ab81719)
  ab81719 at 1/50 dilution staining PLVAP in human tonsil by Immunofluorescence.
• 

  Flow Cytometry-Anti-PLVAP antibody [174/2](ab81719)
  Overlay histogram showing HL60 cells stained with ab81719 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab81719, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HL60 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.