Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
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ab53773 has been referenced in 3 publications.
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All lanes : Anti-PML Protein antibody (ab53773) at 1/500 dilution
Lane 1 : Extracts from A549 cells
Lane 2 : Extracts from A549 cells, plus immunising peptide
Predicted band size : 69 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
ab53773 (1/200) staining PML protein in Hela cells (green). Cells were fixed with methanol, permeabilised with triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.
This image is courtesy of an Abreview submitted by Dr Kirk McManus
Ab53773 staining Human normal colon. Staining is localized to the nucleus.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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