Anti-PML+RARA Fusion antibody (ab43152)
Key features and details
- Rabbit polyclonal to PML+RARA Fusion
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-PML+RARA Fusion antibody -
Description
Rabbit polyclonal to PML+RARA Fusion -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human PML+RARA Fusion aa 500-600 conjugated to keyhole limpet haemocyanin.
(Peptide available asab46898) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab43152 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 98 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 98 kDa. |
Target
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Relevance
PML protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. Retinoic acid, a metabolite of vitamin A, is necessary for normal organogenesis but acts as a teratogen at high levels during embryonic and fetal development. Retinoic acid functions through its interaction with the nuclear protein, retinoic acid receptor (RAR). RAR belongs to the steroid and thyroid hormone superfamily of nuclear receptor proteins which exert their effects by binding to specific DNA response elements, thus regulating gene expression in target cells. RAR exists as three major subtypes: alpha, beta and gamma. -
Cellular localization
Nuclear and Cytoplasmic -
Database links
- Entrez Gene: 5371 Human
- Entrez Gene: 5914 Human
- Omim: 102578 Human
- Omim: 180240 Human
- SwissProt: P10276 Human
- SwissProt: P29590 Human
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Alternative names
- MYL antibody
- NR1B1 antibody
- Nuclear receptor subfamily 1 group B member 1 antibody
see all
Images
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Anti-PML+ RARα Fusion antibody (ab43152) reactivity with reduced NB4 (A and B) and K562 (B) cell lysate. After SDS-PAGE (where 100μg lysate was loaded in A and 20μg lysate in B), membranes were blocked in 5% Milk in PBS + 0.1% Tween for 1h at 25°C before incubation with ab43152 (1:5,000 dilution in 1% Milk PBS + 0.1% Tween) for 16h at 4ºC. Blots was then incubated with an anti-Rabbit HRP-conjugated secondary antibody before developing with ECL.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab43152 has been referenced in 12 publications.
- Qi X et al. HDN-1 induces cell differentiation toward apoptosis in promyelocytic leukemia cells depending on its selective effect on client proteins of Hsp90. Toxicol Appl Pharmacol 417:115459 (2021). PubMed: 33609515
- Zhao M et al. OGP46 Induces Differentiation of Acute Myeloid Leukemia Cells via Different Optimal Signaling Pathways. Front Cell Dev Biol 9:652972 (2021). PubMed: 33748146
- Della Via FI et al. (-)-Epigallocatechin-3-gallate induces apoptosis and differentiation in leukaemia by targeting reactive oxygen species and PIN1. Sci Rep 11:9103 (2021). PubMed: 33907248
- Li S et al. A novel all-trans retinoic acid derivative regulates cell cycle and differentiation of myelodysplastic syndrome cells by USO1. Eur J Pharmacol 906:174199 (2021). PubMed: 34058203
- Kumar S & Tchounwou PB Trisenox Disrupts MDM2-DAXX-HAUSP Complex and Induces Apoptosis in a Mouse Model of Acute Leukemia. J Cancer 11:4373-4383 (2020). PubMed: 32489456