PMN Elastase Human ELISA kit (with color giving dyes) (ab119553)
- Product namePMN Elastase Human ELISA kit (with color giving dyes)
Intra-assay Sample n Mean SD CV% Overall 4.8%
- Tests1 x 96 test
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity1.98 pg/ml
- Range0.16 ng/ml - 10 ng/ml
Sample specific recovery Sample type Average % Range Plasma 104 96% - 110%
- Species reactivityReacts with: Human
- Product overview
ab119553 PMN Elastase Human ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of Human PMN elastase. ab119553 is for research use only and is not for diagnostic or therapeutic procedures.
The human organism reacts with an inflammatory response to attacks of invading pathogens (microorganisms and viruses) or damaged tissue (after accidents or surgery). Polymorphonuclear (PMN) granulocytes play an important role as primary defence cells in this inflammatory reaction. Different bloodstream mediators (cytokines, leukotrienes, complement factors, bacterial endotoxins, clotting and fibrinolysis factors) attract and stimulate these cells to phagocytize and destroy not naturally occurring agents.
PMN granulocytes use proteinases to digest these agents and tissue debris. One of these proteinases is PMN elastase which is localised in the azurophilic granules of the polymorphonuclear granulocytes. During phagocytosis of foreign substances these enzymes are also partially excreted into the extracellular surrounding, where the activity of PMN elastase is regulated by inhibitors (esp. the alpha 1-proteinase inhibitor, alpha 1- PI). An overwhelming release of PMN elastase, however, can exceed the inhibitory potential of the alpha 1-proteinase inhibitor. Thus, enzymatically active PMN elastase, together with simultaneously produced oxidants (O2-radicals, H2O2, OH-radicals), can cause local tissue injury. Due to the bloodstream and lymphatic system, however, alpha 1-PI is delivered subsequently and eventually able to form a complex with all excreted elastase. Therefore, the concentration of the PMN elastase/alpha 1- PI complex correlates with the released PMN elastase and can be used as a measure for the activity of granulocytes during an inflammatory response. Primarily, determinations of PMN elastase find its application in observation of the course of trauma, shock and sepsis. Further indications are the areas of hemodialysis, infections by obstetrics, joint diseases, effusions of sport injuries, intestinal affection, pancreatitis, cystic fibrosis and male adnex affections.
- Tested applicationsSandwich ELISA more details
- Storage instructionsPlease refer to protocols.
Components 1 x 96 tests Adhesive Films 2 units Control high 1 vial Control low 1 vial HRP-Conjugate anti-alpha1-PI polyclonal antibody 1 x 16ml Human PMN Elastase Standard 1 vial Polyclonal anti-human PMN Elastase-coated microwell plate 1 unit Sample Diluent 1 x 50ml Stop Solution 1 x 7ml Substrate Solution 1 x 22ml Wash Buffer Concentrate (10x) 1 x 50ml
Defects in ELANE are the cause of neutropenia severe congenital autosomal dominant type 1 (SCN1) [MIM:202700]. SCN1 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections.
Contains 1 peptidase S1 domain.
- Bone marrow serine proteaseELANEElastase 2 neutrophil
- Elastase neutrophil expressedElastase-2ELNE_HUMANGEgranulocyte derived elastaseHLEHNEHuman leukocyte elastaseLeukocyte elastaseMedullasinNENeutrophil elastasePMN EPMN elastasePolymorphonuclear elastaseSCN1
Our Abpromise guarantee covers the use of ab119553 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
PMN Elastase Human ELISA kit (with color giving dyes) images
Representative standard curve using ab119553.
References for PMN Elastase Human ELISA kit (with color giving dyes) (ab119553)
ab119553 has not yet been referenced specifically in any publications.