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PMN Elastase Human ELISA kit (with color giving dyes) (ab119553)

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Overview

  • Product namePMN Elastase Human ELISA kit (with color giving dyes)
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.8%
  • Tests
    1 x 96 test
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    1.98 pg/ml
  • Range
    0.16 ng/ml - 10 ng/ml
  • Recovery

    104 %

    Sample specific recovery
    Sample type Average % Range
    Plasma 104 96% - 110%

  • Species reactivity
    Reacts with: Human
  • Product overview

    ab119553 PMN Elastase Human ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of Human PMN elastase. ab119553 is for research use only and is not for diagnostic or therapeutic procedures.

     

    The human organism reacts with an inflammatory response to attacks of invading pathogens (microorganisms and viruses) or damaged tissue (after accidents or surgery). Polymorphonuclear (PMN) granulocytes play an important role as primary defence cells in this inflammatory reaction. Different bloodstream mediators (cytokines, leukotrienes, complement factors, bacterial endotoxins, clotting and fibrinolysis factors) attract and stimulate these cells to phagocytize and destroy not naturally occurring agents.

    PMN granulocytes use proteinases to digest these agents and tissue debris. One of these proteinases is PMN elastase which is localised in the azurophilic granules of the polymorphonuclear granulocytes. During phagocytosis of foreign substances these enzymes are also partially excreted into the extracellular surrounding, where the activity of PMN elastase is regulated by inhibitors (esp. the alpha 1-proteinase inhibitor, alpha 1- PI). An overwhelming release of PMN elastase, however, can exceed the inhibitory potential of the alpha 1-proteinase inhibitor. Thus, enzymatically active PMN elastase, together with simultaneously produced oxidants (O2-radicals, H2O2, OH-radicals), can cause local tissue injury. Due to the bloodstream and lymphatic system, however, alpha 1-PI is delivered subsequently and eventually able to form a complex with all excreted elastase. Therefore, the concentration of the PMN elastase/alpha 1- PI complex correlates with the released PMN elastase and can be used as a measure for the activity of granulocytes during an inflammatory response. Primarily, determinations of PMN elastase find its application in observation of the course of trauma, shock and sepsis. Further indications are the areas of hemodialysis, infections by obstetrics, joint diseases, effusions of sport injuries, intestinal affection, pancreatitis, cystic fibrosis and male adnex affections.

  • Tested applicationsSandwich ELISA more details

Properties

  • FunctionModifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis.
  • Tissue specificityBone marrow cells.
  • Involvement in diseaseDefects in ELANE are a cause of cyclic haematopoiesis (CH) [MIM:162800]; also known as cyclic neutropenia. CH is an autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Circulating neutrophils vary between almost normal numbers and zero. During intervals of neutropenia, affected individuals are at risk for opportunistic infection. Monocytes, platelets, lymphocytes and reticulocytes also cycle with the same frequency.
    Defects in ELANE are the cause of neutropenia severe congenital autosomal dominant type 1 (SCN1) [MIM:202700]. SCN1 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections.
  • Sequence similaritiesBelongs to the peptidase S1 family. Elastase subfamily.
    Contains 1 peptidase S1 domain.

    • Target information above from: UniProt accession P08246 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        Bone marrow serine proteaseELANEElastase 2 neutrophil
        Elastase neutrophil expressedElastase-2ELNE_HUMANGEgranulocyte derived elastaseHLEHNEHuman leukocyte elastaseLeukocyte elastaseMedullasinNENeutrophil elastasePMN EPMN elastasePolymorphonuclear elastaseSCN1
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab119553 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      PMN Elastase Human ELISA kit (with color giving dyes) images

      • Sandwich ELISA - PMN Elastase Human ELISA Kit (with color giving dyes) (ab119553)
        Sandwich ELISA - PMN Elastase Human ELISA Kit (with color giving dyes) (ab119553)
        Representative standard curve using ab119553.

      Protocols

      References for PMN Elastase Human ELISA kit (with color giving dyes) (ab119553)

      ab119553 has not yet been referenced specifically in any publications.

      Publishing research using ab119553 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab119553 Submit a Question for ab119553

      Displaying 1 - 8 of 8 results for Abreviews and Q&A

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      Votes: Highest First

      Dear

      Many thanks for your help and support with this. I look forward to hearing from you.

      Kind regards,

      I am sorry for the delay in getting back to you.

      I now have further information to share with you in regards to the Neutrophil Elastase ELISA you are hoping to set up.

      If you would be interested in setting up your own ELISA I have looked...

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      Phone call requesting the standard provided with the PMN Elastase Human ELISA kit (ab119553) to be purchased separately.

      Thank you for contacting us and your interest in our products.

      I am sorry but having looked into it, we are unfortunately not able to provide the standard or high/low samples for the PMN Elastase Human ELISA kit (ab119553) separately.

      We...

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      I am interested in purchasing the capture and detection antibodies and the standard used in this ELISA kit:
      http://www.abcam.com/PMN-Elastase-Human-ELISA-kit-with-color-giving-dyes-ab119553.html

      Will you tell me the catalog numbers for the...

      Read More

      Thank you for contacting us.
      Unfortunately, it is not possible to obtain the standard and detection antibody separately for this kit. Also, the coating antibody is bound on the plates and is not available separately either.
      I am sorry to not be ...

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      I have a question regarding ab119553 Human PMN Elastase ELISA Kit. I noticed that the ELISA is based on detecting human PMN elastase/a1-PI complexes. Does it mean that the standard is also lyophilized PMN elastase/alpha 1-PI complex standard?

      T...

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      Thank you for contacting Abcam regarding ab119553.


      I can confirm that this kit will only recognize the complex of PMN elastase/alpha 1-PI complex standards and also our standard is lyophilized complex.


      I hope this informati...

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      I would like to receive one of those kits, please (8-hydroxy 2 deoxyguanosine ELISA Kit (ab101245)).

      Ihave issued a freekit, 8-hydroxy 2 deoxyguanosine ELISA Kit, ab101245,with the order number 1070428.

      To check the status of the order please contact our Customer Service team and reference this number.

      Again, I apologize for any del...

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      We talked on the phone earlier today and you offered a compensation for the problems that we experienced with 2 of your ELISA kits:

      1. ab108907 - Thrombin-Antithrombin Complex Human ELISA Kit
      2. ab119553 - PMN Elastase Human ELISA Kit...

      Read More

      Thanks for your email.

      As we discussed yesterday, I am happy to provide one kit of your choice free of charge. We currently have 8-hydroxy 2 deoxyguanosine ELISA Kit (ab101245) in stock.. If you'd like I will send one out to you right away.


      To clarify this issue even better, please find a photo that I have enclosed for you below. In the manual (also enclosed), on page 12, you will find the following article:

      2. Reconstitution volume is stated on the label of the
      standard...

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      Thanks for your email and for the attached image. I have forwarded it to our stocks team in the UK where this kit is processed. They should reply tomorrow at which time I will follow up with you.

      I am sorry for the trouble here but I know this w...

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      How much volume should I add to the Standard to produce 10ng/ml for lot GR80860-1?

      Sorry for the inconvenience due to thisimproperly labeled vial.

      I have found that the standard should be resuspended in 2 mlsSample Diluent. This is a lot specific volume.

      I hope this is helpful. Please contact me again if you have any...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"