Anti-PNPLA3 antibody (ab81874)
Key features and details
- Rabbit polyclonal to PNPLA3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PNPLA3 antibody
See all PNPLA3 primary antibodies -
Description
Rabbit polyclonal to PNPLA3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab102097) -
Positive control
- This antibody gave a positive signal in the following lysates: Human Liver Tissue; HepG2 Whole Cell Lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab81874 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 53 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 53 kDa). |
Target
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Relevance
PNPLA3 is a multifunctional enzyme which has both triacylglycerol lipase and acylglycerol O-acyltransferase activities. It contains one patatin domain. -
Cellular localization
Membrane; Single pass type II membrane protein -
Database links
- Entrez Gene: 80339 Human
- Entrez Gene: 116939 Mouse
- Omim: 609567 Human
- SwissProt: Q9NST1 Human
- SwissProt: Q91WW7 Mouse
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Alternative names
- Acylglycerol O acyltransferase antibody
- Adiponutrin antibody
- ADPN antibody
see all
Images
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All lanes : Anti-PNPLA3 antibody (ab81874) at 1 µg/ml
Lane 1 : Human liver tissue lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Predicted band size: 53 kDa
Observed band size: 35,55,70 kDa why is the actual band size different from the predicted?Blocking buffer: 2% BSA
Gel type: MOPS
Exposure Time: 12 minutes
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All lanes : ab81874 at 1 µg/ml
Lane 1 : Human liver
Lane 2 : Human whole cell lysate HepG2
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : ab97080 at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 53 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Additional bands at: 120 kDa, 48 kDa. We are unsure as to the identity of these extra bands.The PNPLA3 sequence contains two glycosylation sites (Swissprot data). The additional band close to the band of interest might be a glycosylated form of PNPLA3. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (16)
ab81874 has been referenced in 16 publications.
- Erb SJ et al. Responsiveness of PNPLA3 and lipid-related transcription factors is dependent upon fatty acid profile in primary bovine hepatocytes. Sci Rep 12:888 (2022). PubMed: 35042927
- Su W et al. Phosphorylation of 17β-hydroxysteroid dehydrogenase 13 at serine 33 attenuates nonalcoholic fatty liver disease in mice. Nat Commun 13:6577 (2022). PubMed: 36323699
- Pralle RS et al. Greater liver PNPLA3 protein abundance in vivo and in vitro supports lower triglyceride accumulation in dairy cows. Sci Rep 11:2839 (2021). PubMed: 33531537
- Mantovani A et al. PNPLA3 I148M gene variant and chronic kidney disease in type 2 diabetic patients with NAFLD: Clinical and experimental findings. Liver Int 40:1130-1141 (2020). PubMed: 32125756
- Yuan S et al. PNPLA3 I148M mediates the regulatory effect of NF-kB on inflammation in PA-treated HepG2 cells. J Cell Mol Med 24:1541-1552 (2020). PubMed: 31793207