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Western blot - PODXL antibody (ab62594)
(enlarge)
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Product Name
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PODXL antibody
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See all PODXL antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to PODXL
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Immunogen
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A region within synthetic peptide: RGGGSGNPTT TIESPKSTKS ADTTTVATST ATAKPNTTSS QNGAEDTTNS, corresponding to N terminal amino acids 109-158 of Human PODXL
BLAST 'RGGGSGNPTT TIESPKSTKS ADTTTVATST ATAKPNTTSS QNGAEDTTNS ' with  or 
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
Note: titre using peptide based assay: 1:312500
WB: Use at a concentration of 5 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HepG2 cell lysate.
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Cellular localization
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Membrane. Single-pass type I membrane protein.
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Research areas
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Cardiovascular >> Vasculature >> Endothelium
Cancer >> Tumor biomarkers >> Other
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Focal Adhesions
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Relevance
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Podocalyxin-like protein (PODXL) is a transmembrane sialomucin that is similar instructure to the well-characterized L-selectin ligand CD34. PCLP has been shown previously to be expressed on the foot processes of podocytes in the kidney glomerulus as well as on vascular endothelium at some sites. PODXL also has a putative role as a diagnostic marker for a number of cancers including pancreatic ductal carcinomas and prostate cancer.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Lyophilised
Add 100 µl of distilled water.
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PODXL antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for PODXL antibody images, references, products related to ab62594 and other tools.
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PODXL antibody images:
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 Western blot - PODXL antibody (ab62594)
PODXL antibody (ab62594) at 5 µg/ml + HepG2 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 55 kDa
Observed band size : 55 kDa
Additional bands at : 34 kDa. We are unsure as to the identity of these extra bands.
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Search PubMed (MEDLINE) for references to PODXL
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PODXL antibody - more information
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Customer reviews (feedback) regarding PODXL antibody |
Customer FAQs regarding PODXL antibody |
Protocols for PODXL antibody |
Price and availability of products related to PODXL antibody |
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PODXL antibody - related products:
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PODXL antibody - other tools:
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Contact Abcam with a Technical Enquiry about PODXL antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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