Recombinant Anti-RPB3 antibody [EPR13294(B)] (ab182150)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13294(B)] to RPB3
- Suitable for: IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-RPB3 antibody [EPR13294(B)]
See all RPB3 primary antibodies -
Description
Rabbit monoclonal [EPR13294(B)] to RPB3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human ovarian carcinoma tissue; HeLa, Jurkat, 293T and Human fetal kidney lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13294(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab182150 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/10000 - 1/50000. Detects a band of approximately 33 kDa (predicted molecular weight: 31 kDa).
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Notes |
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IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/10000 - 1/50000. Detects a band of approximately 33 kDa (predicted molecular weight: 31 kDa). |
Target
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Function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft. -
Sequence similarities
Belongs to the archaeal RpoD/eukaryotic RPB3 RNA polymerase subunit family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5432 Human
- Entrez Gene: 20021 Mouse
- Entrez Gene: 361365 Rat
- Omim: 180663 Human
- SwissProt: P19387 Human
- SwissProt: P97760 Mouse
- Unigene: 79402 Human
- Unigene: 292492 Mouse
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Alternative names
- DNA directed RNA polymerase II 33 kDa polypeptide antibody
- DNA directed RNA polymerase II subunit C antibody
- DNA directed RNA polymerase II subunit RPB3 antibody
see all
Images
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All lanes : Anti-RPB3 antibody [EPR13294(B)] (ab182150) at 1/50000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Human fetal kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 31 kDa -
Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling RPB3 with ab182150 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab182150 has been referenced in 2 publications.
- Ding H et al. Differentially expressed mRNAs and their upstream miR-491-5p in patients with coronary atherosclerosis as well as the function of miR-491-5p in vascular smooth muscle cells. Korean J Physiol Pharmacol 26:183-193 (2022). PubMed: 35477546
- Mayfield JE et al. Tyr1 phosphorylation promotes phosphorylation of Ser2 on the C-terminal domain of eukaryotic RNA polymerase II by P-TEFb. Elife 8:N/A (2019). PubMed: 31385803