Anti-POLR2E antibody (ab68085)

Overview

• Product nameAnti-POLR2E antibodySee all POLR2E primary antibodies ...
• Description
  Mouse polyclonal to POLR2E
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  POLR2E (1 a.a. ~ 210 a.a) full-length human protein.

• Positive controlPOLR2E transfected 293T cell lysate

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 1X PBS, pH 7.2
• Concentration information loading...
• PurityProtein G purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Transcription
  • Polymerase associated factors
  • Pol II Transcription
  • Polymerase

  • Epigenetics and Nuclear Signaling
  • Transcription
  • RNA polymerase

Applications

Target

• FunctionDNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process.
• Sequence similaritiesBelongs to the archaeal rpoH/eukaryotic RPB5 RNA polymerase subunit family.
• Cellular localizationNucleus.

Target information above from: UniProt accession P19388 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5434 Human
  • Omim: 180664 Human
  • SwissProt: P19388 Human
  • Unigene: 24301 Human

• Alternative names
  and III subunit ABC1 antibodyand III subunit RPABC1 antibodyDNA directed RNA polymerase II 23 kda polypeptide antibody

  DNA directed RNA polymerase II 23 kDa polypeptide antibodyDNA directed RNA polymerase II polypeptide E antibodyDNA directed RNA polymerase II subunit E antibodyDNA directed RNA polymerases I II and III subunit RPABC1 antibodyDNA-directed RNA polymerase II 23 kDa polypeptide antibodyDNA-directed RNA polymerase II subunit E antibodyDNA-directed RNA polymerases I antibodyhRPB25 antibodyhsRPB5 antibodyII antibodyPolr2e antibodyPolymerase (RNA) II (DNA directed) polypeptide E (25kD) antibodyPolymerase (RNA) II (DNA directed) polypeptide E, 25kDa antibodyRNA polymerases I antibodyRNA polymerases I, II, and III subunit ABC1 antibodyRPAB1_HUMAN antibodyRPABC1 antibodyRPB5 antibodyRPB5 homolog antibodyXAP4 antibody

  see all

Anti-POLR2E antibody images

• 

  Western blot - POLR2E antibody (ab68085)
  All lanes : Anti-POLR2E antibody (ab68085) at 1/500 dilution
  
  Lane 1 : POLR2E transfected 293T cell lysate
  Lane 2 : non transfected 293T cell lysate
  
  Lysates/proteins at 25 µg per lane.
  
  Secondary
  Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
  
  Predicted band size : 25 kDa
  Observed band size : 26 kDa (why is the actual band size different from the predicted?)