Anti-PON1 antibody [2A9] (ab114007)

Overview

• Product nameAnti-PON1 antibody [2A9]See all PON1 primary antibodies ...
• Description
  Mouse monoclonal [2A9] to PON1
• Tested applicationsWB, Flow Cyt more details
• Species reactivity
  Reacts with: Human, Monkey
  
• Immunogen

  Recombinant full length Human PON1 (NP_000437) produced in HEK293T cells

• Positive controlHEK293T cell lysate transfected with pCMV6-ENTRY PON1; HEK293T cells transfected with PON1 plasmid; HeLa cells; Jurkat cells; HeLa, SVT2, COS7 cell extracts
• General notesDilute in PBS (pH7.3) before use.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferpH: 7.30
  Preservative: 0.02% Sodium azide
  Constituents: 1% BSA, 50% Glycerol, 48% PBS
• Concentration information loading...
• PurityProtein G purified
• Purification notesab114007 was purified from Mouse ascites fluids by affinity chromatography.
• Clonality Monoclonal
• Clone number2A9
• IsotypeIgG1
• Research Areas
  • Metabolism
  • Types of disease
  • Heart disease

  • Metabolism
  • Types of disease
  • Neurodegenerative disease

  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Types of disease
  • Diabetes

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Hydrolysis

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Drug metabolism

  • Metabolism
  • Pathways and Processes
  • Redox metabolism
  • Oxidative stress

  • Signal Transduction
  • Metabolism
  • Drug metabolism

  • Signal Transduction
  • Metabolism
  • Lipid metabolism

  • Cardiovascular
  • Lipids / Lipoproteins
  • Lipoproteins/Apolipoproteins

  • Cardiovascular
  • Lipids / Lipoproteins
  • Lipid Metabolism
  • Hydrolysis

  • Cardiovascular
  • Atherosclerosis
  • Diabetes associated

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Parkinson's disease
  • Other

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Other

  • Cell Biology
  • Other Antibodies
  • Oxidative Stress

Applications

Target

• FunctionHydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation.
• Tissue specificityPlasma, associated with HDL (at protein level). Expressed in liver, but not in heart, brain, placenta, lung, skeletal muscle, kidney or pancreas.
• Involvement in diseaseGenetic variation in PON1 is associated with susceptibility to microvascular complications of diabetes type 5 (MVCD5) [MIM:612633]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Homozygosity for the Leu-54 allele is strongly associated with the development of retinal disease in diabetic patients.
• Sequence similaritiesBelongs to the paraoxonase family.
• Post-translational
  modificationsGlycosylated.
  The signal sequence is not cleaved.
  Present in two forms, form B contains a disulfide bond, form A does not.
• Cellular localizationSecreted > extracellular space.

Target information above from: UniProt accession P27169 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5444 Human
  • Omim: 168820 Human
  • SwissProt: P27169 Human
  • Unigene: 370995 Human

• Alternative names
  A esterase 1 antibodyA-esterase 1 antibodyAromatic esterase 1 antibody

  Arylesterase B type antibodyESA antibodyEsterase A antibodyK 45 antibodyK-45 antibodyMVCD5 antibodyParaoxonase 1 antibodyParaoxonase antibodyParaoxonase B type antibodyParaoxonase1 antibodyPON 1 antibodyPON antibodyPON1 antibodyPON1_HUMAN antibodySerum aryldiakylphosphatase antibodySerum aryldialkylphosphatase 1 antibodySerum paraoxonase/arylesterase 1 antibody

  see all

Anti-PON1 antibody [2A9] images

• 

  Western blot - Anti-PON1 antibody [2A9] (ab114007)
  All lanes : Anti-PON1 antibody [2A9] (ab114007) at 1/500 dilution
  
  Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
  Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY PON1 cDNA
  
  Lysates/proteins at 5 µg per lane.
  
  
  Predicted band size : 40 kDa
  
• 

  Western blot - Anti-PON1 antibody [2A9] (ab114007)
  All lanes : Anti-PON1 antibody [2A9] (ab114007) at 1/500 dilution
  
  Lane 1 : HepG2 cell extract
  Lane 2 : HeLa cell extract
  Lane 3 : SVT2 cell extract
  Lane 4 : A549 cell extract
  Lane 5 : COS7 cell extract
  Lane 6 : Jurkat cell extract
  Lane 7 : MDCK cell extract
  Lane 8 : PC12 cell extract
  Lane 9 : MCF7 cell extract
  
  Lysates/proteins at 35 µg per lane.
  
  
  Predicted band size : 40 kDa
  
• 

  Flow Cytometry - Anti-PON1 antibody [2A9] (ab114007)
  ab114007 at 1/100 staining PON1 in HEK293T cells transfected with PON1 overexpression plasmid (red) or empty vector control (blue), and analyzed by flow cytometry.
• 

  Flow Cytometry - Anti-PON1 antibody [2A9] (ab114007)
  ab114007 at 1/100 staining PON1 in HeLa cells (red) compared to a nonspecific negative control (blue), and analyzed by flow cytometry.
• 

  Flow Cytometry - Anti-PON1 antibody [2A9] (ab114007)
  ab114007 at 1/100 staining PON1 in Jurkat cells (red) compared to a nonspecific negative control (blue), and analyzed by flow cytometry.