Anti-PON1 antibody [2A9] (ab114007)

Overview

  • Product nameAnti-PON1 antibody [2A9]See all PON1 primary antibodies ...
  • Description
    Mouse monoclonal [2A9] to PON1
  • Tested applicationsWB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human, Monkey
  • Immunogen

    Recombinant full length Human PON1 (NP_000437) produced in HEK293T cells

  • Positive control
    • HEK293T cell lysate transfected with pCMV6-ENTRY PON1; HEK293T cells transfected with PON1 plasmid; HeLa cells; Jurkat cells; HeLa, SVT2, COS7 cell extracts
  • General notesDilute in PBS (pH7.3) before use.

Applications

Our Abpromise guarantee covers the use of ab114007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 40 kDa.
Flow Cyt 1/100.

Target

  • FunctionHydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation.
  • Tissue specificityPlasma, associated with HDL (at protein level). Expressed in liver, but not in heart, brain, placenta, lung, skeletal muscle, kidney or pancreas.
  • Involvement in diseaseGenetic variation in PON1 is associated with susceptibility to microvascular complications of diabetes type 5 (MVCD5) [MIM:612633]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Homozygosity for the Leu-54 allele is strongly associated with the development of retinal disease in diabetic patients.
  • Sequence similaritiesBelongs to the paraoxonase family.
  • Post-translational
    modifications
    Glycosylated.
    The signal sequence is not cleaved.
    Present in two forms, form B contains a disulfide bond, form A does not.
  • Cellular localizationSecreted > extracellular space.
  • Information by UniProt
  • Database links
  • Alternative names
    • A esterase 1 antibody
    • A-esterase 1 antibody
    • Aromatic esterase 1 antibody
    • Arylesterase 1 antibody
    • Arylesterase B type antibody
    • ESA antibody
    • Esterase A antibody
    • K 45 antibody
    • K-45 antibody
    • MVCD5 antibody
    • Paraoxonase 1 antibody
    • Paraoxonase antibody
    • Paraoxonase B type antibody
    • Paraoxonase, plasma antibody
    • Paraoxonase1 antibody
    • PON 1 antibody
    • PON antibody
    • PON1 antibody
    • PON1_HUMAN antibody
    • Serum aryldiakylphosphatase antibody
    • Serum aryldialkylphosphatase 1 antibody
    • Serum paraoxonase/arylesterase 1 antibody
    see all

Anti-PON1 antibody [2A9] images

  • All lanes : Anti-PON1 antibody [2A9] (ab114007) at 1/500 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY PON1 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 40 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC210356)
  • All lanes : Anti-PON1 antibody [2A9] (ab114007) at 1/500 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HeLa cell extract
    Lane 3 : SVT2 cell extract
    Lane 4 : A549 cell extract
    Lane 5 : COS7 cell extract
    Lane 6 : Jurkat cell extract
    Lane 7 : MDCK cell extract
    Lane 8 : PC12 cell extract
    Lane 9 : MCF7 cell extract

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 40 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC210356)
  • ab114007 at 1/100 staining PON1 in HEK293T cells transfected with PON1 overexpression plasmid (red) or empty vector control (blue), and analyzed by flow cytometry.
  • ab114007 at 1/100 staining PON1 in HeLa cells (red) compared to a nonspecific negative control (blue), and analyzed by flow cytometry.
  • ab114007 at 1/100 staining PON1 in Jurkat cells (red) compared to a nonspecific negative control (blue), and analyzed by flow cytometry.

References for Anti-PON1 antibody [2A9] (ab114007)

ab114007 has not yet been referenced specifically in any publications.

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In der Tat kann es möglich sein, dass die Antikörper ab114007 und ab114021 für Sandwich-ELISA (sELISA) geeignet sind. Unseres Wissens nach wurden die beiden PON1-Antikö...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"