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Overview

  • Product nameAnti-PON1 antibodySee all PON1 primary antibodies ...
  • Description
    Rabbit polyclonal to PON1
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human PON1.

    Read Abcam's proprietary immunogen policy

  • Positive controlThis antibody gave a positive signal in Human Liver tissue lysate as well as PON1 recombinant protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab123558 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).

Target

  • FunctionHydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation.
  • Tissue specificityPlasma, associated with HDL (at protein level). Expressed in liver, but not in heart, brain, placenta, lung, skeletal muscle, kidney or pancreas.
  • Involvement in diseaseGenetic variation in PON1 is associated with susceptibility to microvascular complications of diabetes type 5 (MVCD5) [MIM:612633]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Homozygosity for the Leu-54 allele is strongly associated with the development of retinal disease in diabetic patients.
  • Sequence similaritiesBelongs to the paraoxonase family.
  • Post-translational
    modifications    Glycosylated.
    The signal sequence is not cleaved.
    Present in two forms, form B contains a disulfide bond, form A does not.
  • Cellular localizationSecreted > extracellular space.

    • Target information above from: UniProt accession P27169 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      A esterase 1 antibodyA-esterase 1 antibodyAromatic esterase 1 antibody
      Arylesterase B type antibodyESA antibodyEsterase A antibodyK 45 antibodyK-45 antibodyMVCD5 antibodyParaoxonase 1 antibodyParaoxonase antibodyParaoxonase B type antibodyParaoxonase1 antibodyPON 1 antibodyPON antibodyPON1 antibodyPON1_HUMAN antibodySerum aryldiakylphosphatase antibodySerum aryldialkylphosphatase 1 antibodySerum paraoxonase/arylesterase 1 antibody
    see all

Anti-PON1 antibody images

  • Western blot - Anti-PON1 antibody (ab123558)
    Western blot - Anti-PON1 antibody (ab123558)
    All lanes : Anti-PON1 antibody (ab123558) at 1 µg/ml

    Lane 1 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889) at 10 µg
    Lane 2 : PON1 protein (ab53376) at 0.1 µg

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 39 kDa
    Observed band size : 39 kDa


    Exposure time : 30 seconds

References for Anti-PON1 antibody (ab123558)

ab123558 has not yet been referenced specifically in any publications.

Publishing research using ab123558 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

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Votes: Highest First

Dear Technical,



My customer is interested in working with anti-PON1 antibody on rat tissue using IHC-P application.

Would you be able to offer her the testing program if she wishes to test ab123558 using IHC-P method?

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I am very pleased to hear your customer would like to test ab123558 in IHC-P and rat.
I strongly suggest to also run a WB with the rat samples since IHC-P and rat are both untested.
Please provide your customer's email address and the institute ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"