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Western blot - PON1 antibody (ab40775)
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Immunohistochemistry (Frozen sections) - PON1 antibody (ab40775)
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Product Name
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PON1 antibody
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See all PON1 antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to PON1
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Rat PON1. (Peptide available as ab41534.)
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Reacts with
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Ms, Rat
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Predicted to react
(species key)
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Hu
(Due to sequence homology)
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Tested applications
(see key)
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IHC-FoFr, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-FoFr: Use at an assay dependent concentration.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 40 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in mouse liver lysate.
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Cellular localization
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Secreted: Extracellular.
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Research areas
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Cardiovascular >> Atherosclerosis >> Diabetes associated
Cell Biology >> Other Antibodies >> Oxidative Stress
Signal Transduction >> Metabolism >> Drug metabolism
Signal Transduction >> Metabolism >> Lipid metabolism
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Hydrolysis
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
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Relevance
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The paraoxonase (PON) gene family in humans has three members, PON1, PON2, and PON3, aligned next to each other on the long arm of chromosome
7q21.3-22.1. PON1 is synthesized primarily in the liver, and a portion is secreted into the plasma, where it is associated with high density lipoproteins (HDL). PON1 hydrolyzes the active metabolites of several other organophosphorus insecticides (e.g., chlorpyrifos oxon, diazoxon), as well as nerve agents such as sarin, soman and VX (lethal nerve agent). The PON1 polymorphism may be responsible for neurodegeneration and is considered to be an independent risk factor for Parkinson’s disease.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PON1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for PON1 antibody images, references, products related to ab40775 and other tools.
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PON1 antibody images:
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 Western blot - PON1 antibody (ab40775)
PON1 antibody (ab40775) at 1 µg/ml + Liver (Mouse) Tissue Lysate - adult normal tissue at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 40 kDa
Observed band size : 43 kDa (why is the actual band size different from the predicted?)
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Immunohistochemistry (Frozen sections) - PON1 antibody (ab40775)
ab40775 staining of PON1 in rat liver sections. Frozen rat liver sections were fixed with PFA and incubated for 18 hours with ab40775 (1/1000). A rabbit IgG antibody (ab60314) was used as a secondary. The right panel shows background staining obtained in absence of primary antibody, while the left panel shows the staining obtained in the presence of ab40775. This image is courtesy of an Abreview submitted by Dr Sophie Pezet
See Abreview
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Search PubMed (MEDLINE) for references to PON1
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PON1 antibody - more information
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Customer reviews (feedback) regarding PON1 antibody |
Customer FAQs regarding PON1 antibody |
Protocols for PON1 antibody |
Price and availability of products related to PON1 antibody |
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PON1 antibody - related products:
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PON1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about PON1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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