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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
Overview
Product name
PON1 protein
Protein description
Recombinant full length PON1 protein (Human) with an amino-terminal hexahistidine tag.
Expression host
E. coli
Properties
Purity
>95% by SDS-PAGE
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol, 20mM Tris, pH 8
Concentration
Concentration information loading...
Research areas
Cardiovascular >> Atherosclerosis >> Diabetes associated
Cell Biology >> Other Antibodies >> Oxidative Stress
Signal Transduction >> Metabolism >> Drug metabolism
Signal Transduction >> Metabolism >> Lipid metabolism
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Cardiovascular >> Lipids / Lipoproteins >> Lipoproteins/Apolipoproteins
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Hydrolysis
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab53376 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent concentration. (Ab53376 can be used as a WB positive control in conjunction with ab24261.)
SDS-PAGE: Use at an assay dependent dilution.
Protein info
Function
Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation.
Tissue specificity
Plasma, associated with HDL (at protein level). Expressed in liver, but not in heart, brain, placenta, lung, skeletal muscle, kidney or pancreas.
Involvement in disease
Genetic variation in PON1 is associated with susceptibility to microvascular complications of diabetes type 5 (MVCD5) [MIM:612633]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Homozygosity for the Leu-54 allele is strongly associated with the development of retinal disease in diabetic patients.
Sequence similarities
Belongs to the paraoxonase family.
Post-translational
modifications
Glycosylated.
The signal sequence is not cleaved.
Present in two forms, form B contains a disulfide bond, form A does not.
Cellular localization
Secreted > extracellular space.
Target information above from: UniProt accessionP27169
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
PON1 protein images:
Western blot
Anti-PON1 antibody [17A12] (ab24261) at 1/500 dilution + PON1 protein (ab53376) at 0.01 µg
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/500 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 1 minute
References for PON1 protein (ab53376)
ab53376 has not yet been referenced specifically in any publications.
Publishing research using ab53376? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"