Overview
- Product nameAnti-PON2 antibodySee all PON2 primary antibodies ...
- DescriptionRabbit polyclonal to PON2
- Tested applicationsIHC-P, WB, ICC/IF more details
- Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Dog - Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human PON2.
(Peptide available as ab41542.)
- Positive controlThis antibody gave a positive signal in the following Human whole cell lysates: Caco 2; HeLa - Hydroxyurea Treated (48hr, 1µM). HeLa cell line in IF.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab40969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IHC-P | IHC-P: 1/2000. |
| WB | WB: Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).Can be blocked with PON2 peptide (ab41542). |
| ICC/IF | ICC/IF: Use a concentration of 5 µg/ml. |
Target
- FunctionCapable of hydrolyzing lactones and a number of aromatic carboxylic acid esters. Has antioxidant activity. Is not associated with high density lipoprotein. Prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis.
- Tissue specificityWidely expressed with highest expression in liver, lung, placenta, testis and heart.
- Sequence similaritiesBelongs to the paraoxonase family.
- Post-translational
modificationsThe signal sequence is not cleaved. - Cellular localizationMembrane.
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Database links
- Entrez Gene: 395830 Chicken
- Entrez Gene: 281417 Cow
- Entrez Gene: 403855 Dog
- Entrez Gene: 5445 Human
- Entrez Gene: 330260 Mouse
- Entrez Gene: 296851 Rat
- Omim: 602447 Human
- SwissProt: Q90952 Chicken
- SwissProt: Q58DS7 Cow
- SwissProt: P54832 Dog
- SwissProt: Q15165 Human
- SwissProt: Q62086 Mouse
- SwissProt: Q6AXM8 Rat
- Unigene: 727553 Human
- Unigene: 126984 Mouse
- Unigene: 460999 Mouse
- Unigene: 1100 Rat
see all
Target information above from: UniProt accession
Q15165
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- A esterase 2 antibodyA-esterase 2 antibodyAromatic esterase 2 antibody
- Paraoxonase 2 antibodyParaoxonase nirs antibodyPON 2 antibodyPON2 antibodyPON2_HUMAN antibodySerum aryldialkylphosphatase 2 antibodySerum paraoxonase/arylesterase 2 antibody
see all
Anti-PON2 antibody images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PON2 antibody (ab40969)Carl Hobbs, King`s College London, United KingdomImmunohistochemistical detection of PON2 antibody (ab40969) on formaldehyde fixed paraffin-embedded mosue kidney and lung sectionsAntigen retrieval step: Heat mediated. Buffer: Citric acid pH6. Permeabilization: None. Blocking step: 1% BSA for 10 mins @ 21°C. Primary antibody incubated at 1/2000 for 2 hours @ 21°C in TBS/BSA/azide. Secondary antibody: anti Rabbit IgG Conjugated to Biotin (1/200). Patterns of immunostaining are produced that appear to be very similar to those seen on the HPA website, for these tissues. In the submitted composite image, both tissues show intense labelling. In the upper image of kidney cortico-medullary junction, apical membrane positivity is observed in proximal/distal tubules. In the same tubules globular intra-cytoplasmic positivity is also observed. In the lower image of lung, positivity is evident in the simple columnar epithelium of the bronchioles and also in what appear to be alveolar macrophages. This antibody should be diluted further, under the conditions used.
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Western blot - Anti-PON2 antibody (ab40969)All lanes : Anti-PON2 antibody (ab40969) at 1 µg/ml
Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 2 : Hela Whole Cell Lysate - Hydroxyurea Treated (48hr, 1uM)
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 40 kDa
Observed band size : 40 kDa
Additional bands at : 45 kDa (possible glycosylated form),55 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 150 seconds -
Western blot - Anti-PON2 antibody (ab40969)Anti-PON2 antibody (ab40969) at 1 µg/ml +PON2 protein (ab53379) at 0.01 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 30 seconds -
Immunocytochemistry/ Immunofluorescence - Anti-PON2 antibody (ab40969)ICC/IF image of ab40969 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40969, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-PON2 antibody (ab40969)
This product has been referenced in:
- Giordano G et al. Gender differences in brain susceptibility to oxidative stress are mediated by levels of paraoxonase-2 expression. Free Radic Biol Med 58:98-108 (2013). WB ; Mouse, Human . Read more (PubMed: 23376469) »
