Anti-PON3 antibody (ab42322)

Overview

• Product nameAnti-PON3 antibodySee all PON3 primary antibodies ...
• Description
  Rabbit polyclonal to PON3
• Tested applicationsICC/IF, IHC-P, WB more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human PON3.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab42321.)

• Positive controlThis antibody gave a positive signal in the following tissue lysates: Human Skeletal Muscle, Mouse Liver, Rat Liver and Rat Skeletal Muscle. IF/ICC: HepG2 cell line.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Types of disease
  • Neurodegenerative disease

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Drug metabolism

  • Signal Transduction
  • Metabolism
  • Drug metabolism

  • Cardiovascular
  • Lipids / Lipoproteins
  • Lipoproteins/Apolipoproteins

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Parkinson's disease
  • Other

Applications

Target

• FunctionHas low activity towards the organophosphate paraxon and aromatic carboxylic acid esters. Rapidly hydrolyzes lactones such as statin prodrugs (e.g. lovastatin). Hydrolyzes aromatic lactones and 5- or 6-member ring lactones with aliphatic substituents but not simple lactones or those with polar substituents.
• Sequence similaritiesBelongs to the paraoxonase family.
• Post-translational
  modificationsThe signal sequence is not cleaved.
• Cellular localizationSecreted > extracellular space.

Target information above from: UniProt accession Q15166 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5446 Human
  • Entrez Gene: 269823 Mouse
  • Entrez Gene: 312086 Rat
  • Omim: 602720 Human
  • SwissProt: Q15166 Human
  • SwissProt: Q62087 Mouse
  • SwissProt: Q68FP2 Rat
  • Unigene: 440967 Human

  • Unigene: 9122 Mouse
  • Unigene: 16469 Rat

  see all

• Alternative names
  2810004E20 antibodyAI786302 antibodyMGC95026 antibody

  paraoxonase 3 antibodyparaoxonase3 antibodyPon3 antibodyPON3_HUMAN antibodySerum paraoxonase/lactonase 3 antibody

  see all

Anti-PON3 antibody images

• 

  Immunocytochemistry/ Immunofluorescence - Anti-PON3 antibody (ab42322)

  ICC/IF image of ab42322 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42322, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• 

  Western blot - PON3 antibody (ab42322)
  All lanes : Anti-PON3 antibody (ab42322) at 1 µg/ml
  
  Lane 1 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)
  Lane 2 : Liver (Mouse) Tissue Lysate - normal tissue
  Lane 3 : Liver (Rat) Tissue Lysate
  Lane 4 : Skeletal Muscle (Rat) Tissue Lysate - normal tissue (ab29376)
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size : 40 kDa
  Observed band size : 43 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 49 kDa. We are unsure as to the identity of these extra bands.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON3 antibody (ab42322)
  IHC image of ab42322 staining PON3 in Human liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab42322, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.