Anti-PON3 antibody (ab42322)
- Product nameAnti-PON3 antibodySee all PON3 primary antibodies ...
- DescriptionRabbit polyclonal to PON3
- Tested applicationsICC/IF, IHC-P, WB more details
- Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human PON3.
(Peptide available as ab42321.)
- Positive controlThis antibody gave a positive signal in the following tissue lysates: Human Skeletal Muscle, Mouse Liver, Rat Liver and Rat Skeletal Muscle. IF/ICC: HepG2 cell line.
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
- Concentration information loading...
- PurityImmunogen affinity purified
- Clonality Polyclonal
Our Abpromise guarantee covers the use of ab42322 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||ICC/IF: Use a concentration of 5 µg/ml.|
|IHC-P||IHC-P: Use a concentration of 1 µg/ml.|
|WB||WB: Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 40 kDa).Can be blocked with PON3 peptide (ab42321).|
- FunctionHas low activity towards the organophosphate paraxon and aromatic carboxylic acid esters. Rapidly hydrolyzes lactones such as statin prodrugs (e.g. lovastatin). Hydrolyzes aromatic lactones and 5- or 6-member ring lactones with aliphatic substituents but not simple lactones or those with polar substituents.
- Sequence similaritiesBelongs to the paraoxonase family.
modificationsThe signal sequence is not cleaved.
- Cellular localizationSecreted > extracellular space.
- 2810004E20 antibodyAI786302 antibodyMGC95026 antibody
- paraoxonase 3 antibodyparaoxonase3 antibodyPon3 antibodyPON3_HUMAN antibodySerum paraoxonase/lactonase 3 antibody
Anti-PON3 antibody images
ICC/IF image of ab42322 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42322, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-PON3 antibody (ab42322) at 1 µg/ml
Lane 1 :
Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)
Lane 2 : Liver (Mouse) Tissue Lysate - normal tissue
Lane 3 : Liver (Rat) Tissue Lysate
Lane 4 :
Skeletal Muscle (Rat) Tissue Lysate - normal tissue (ab29376)
Lysates/proteins at 10 µg per lane.
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 40 kDa
Observed band size : 43 kDa (why is the actual band size different from the predicted?)
Additional bands at : 49 kDa. We are unsure as to the identity of these extra bands.
IHC image of ab42322 staining PON3 in Human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab42322, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-PON3 antibody (ab42322)
This product has been referenced in:
- Stone R et al. Identification of genes correlated with early-stage bladder cancer progression. Cancer Prev Res (Phila Pa) 3:776-86 (2010). IHC-P ; Mouse . Read more (PubMed: 20501863) »