Anti-PP2A-alpha antibody (ab106262)
Key features and details
- Rabbit polyclonal to PP2A-alpha
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
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Overview
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Product name
Anti-PP2A-alpha antibody
See all PP2A-alpha primary antibodies -
Description
Rabbit polyclonal to PP2A-alpha -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106262 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 36 kDa.
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IHC-P | (1) |
Use a concentration of 1 - 2 µg/ml. Boiling the sections is required.
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 36 kDa. |
IHC-P
Use a concentration of 1 - 2 µg/ml. Boiling the sections is required. |
Target
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Function
PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen. -
Sequence similarities
Belongs to the PPP phosphatase family. PP-1 subfamily. -
Post-translational
modificationsReversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle.
Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation. -
Cellular localization
Cytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2. - Information by UniProt
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Database links
- Entrez Gene: 5515 Human
- Entrez Gene: 19052 Mouse
- Entrez Gene: 24672 Rat
- Omim: 176915 Human
- SwissProt: P67775 Human
- SwissProt: P63330 Mouse
- SwissProt: P63331 Rat
- Unigene: 105818 Human
see all -
Alternative names
- PP2A alpha antibody
- PP2A-alpha antibody
- PP2AA_HUMAN antibody
see all
Images
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All lanes : Anti-PP2A-alpha antibody (ab106262) at 0.5 µg/ml
Lane 1 : Rat kidney tissue Lysate
Lane 2 : Rat Liver tissue Lysate
Lane 3 : Human Placenta tissue Lysate
Lane 4 : Mouse Kidney tissue Lysate
Lane 5 : A431 Whole Cell Lysate
Lane 6 : HeLa Whole Cell Lysate
Lane 7 : 293T Whole Cell Lysate
Lane 8 : NIH Whole Cell Lysate
Lane 9 : PC12 Whole Cell Lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 36 kDa -
Cytoplasmic staining of PP2A in a formalin fixed, paraffin embedded section of human mammary cancer, using ab106262 at a concentration of 2µg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab106262 has been referenced in 10 publications.
- Gao R et al. Protein phosphatase 2A catalytic subunit β suppresses PMA/ionomycin-induced T-cell activation by negatively regulating PI3K/Akt signaling. FEBS J 289:4518-4535 (2022). PubMed: 35068054
- Liang J et al. The effect of PPP2CA expression on the prognosis of patients with hepatocellular carcinoma and its molecular biological characteristics. J Gastrointest Oncol 12:3008-3021 (2021). PubMed: 35070426
- Liang Y et al. PP2Acα promotes macrophage accumulation and activation to exacerbate tubular cell death and kidney fibrosis through activating Rap1 and TNFα production. Cell Death Differ 28:2728-2744 (2021). PubMed: 33934104
- Lu Q et al. Complement factor B in high glucose-induced podocyte injury and diabetic kidney disease. JCI Insight 6:N/A (2021). PubMed: 34622800
- Yang CL et al. Potential Role and Clinical Value of PPP2CA in Hepatocellular Carcinoma. J Clin Transl Hepatol 9:661-671 (2021). PubMed: 34722181
- Mazhar S et al. Challenges and Reinterpretation of Antibody-Based Research on Phosphorylation of Tyr307 on PP2Ac. Cell Rep 30:3164-3170.e3 (2020). PubMed: 32130915
- Leonard D et al. Selective PP2A Enhancement through Biased Heterotrimer Stabilization. Cell 181:688-701.e16 (2020). PubMed: 32315618
- Stafman LL et al. PP2A activation alone and in combination with cisplatin decreases cell growth and tumor formation in human HuH6 hepatoblastoma cells. PLoS One 14:e0214469 (2019). PubMed: 30969990
- Umesalma S et al. RABL6A inhibits tumor-suppressive PP2A/AKT signaling to drive pancreatic neuroendocrine tumor growth. J Clin Invest 130:N/A (2019). PubMed: 30721156
- Yi H et al. Isosteviol Protects Free Fatty Acid- and High Fat Diet-Induced Hepatic Injury via Modulating PKC-ß/p66Shc/ROS and Endoplasmic Reticulum Stress Pathways. Antioxid Redox Signal 30:1949-1968 (2019). PubMed: 30484323