![Western blot - PP2A alpha (phospho Y307) antibody [E155] (ab32104) image](#WB PP2A alpha (phospho Y307).jpg)
![- PP2A alpha (phospho Y307) antibody [E155] (ab32104) image](#IHC PP2A alpha (phospho Y307).jpg)
 image](#PP2A-alpha-Primary-antibodies-ab32104-1.jpg)
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Product name
Anti-PP2A alpha (phospho Y307) antibody [E155]
See all PP2A alpha products (11) ...
Description
Rabbit monoclonal [E155] to PP2A alpha (phospho Y307)
Specificity
ab32104 recognises only PP2A alpha phosphorylated on Tyrosine 307.
Tested applications
WB, IHC-P, ICC, IP, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Tyr307 of human PP2A alpha catalitic subunit.
Positive control
Human brain tissue WB: lysate of A431 cells (serum starved overnight) treated with EGF (100ng/ml) for 5-10 minutes at 37C (BD Biosciences).
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
E155
Isotype
IgG
Research areas
Cancer >> Cell cycle >> Kinases/phosphatases >> Phosphatases
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Phosphatases
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Phosphatases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32104 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/5000. Predicted molecular weight: 35 kDa.
IHC-P: 1/50.
ICC
ICC: 1/50.ICC: 1/50.
IP
IP: 1/100.IP: 1/100.
ICC/IF: Use a concentration of 5 µg/ml.
Application notes
Is unsuitable for or Flow Cyt.
Target
Function
PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen.
Sequence similarities
Belongs to the PPP phosphatase family. PP-1 subfamily.
Post-translational
modifications
Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle.
Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
Cellular localization
Cytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2.
Target information above from: UniProt accessionP67775
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Medium tumour antigen associated 61kDa protein antibody
- MGC786 antibody
- PP2A alpha antibody
see all
Database links
- Entrez Gene: 5515 Human
- Entrez Gene: 19052 Mouse
- Entrez Gene: 24672 Rat
- Omim: 176915 Human
- SwissProt: P67775 Human
- SwissProt: P63330 Mouse
- SwissProt: P63331 Rat
- Unigene: 105818 Human
see all
Anti-PP2A alpha (phospho Y307) antibody [E155] images:
Western blot - PP2A alpha (phospho Y307) antibody [E155] (ab32104)
![Western blot - PP2A alpha (phospho Y307) antibody [E155] (ab32104)](/ps/datasheet/Images/32/ab32104/WB PP2A alpha (phospho Y307).jpg)
All lanes : Anti-PP2A alpha (phospho Y307) antibody [E155] (ab32104) at 1/5000 dilution
Lane 1 : untreated A431 cell lysate
Lane 2 : EGF treated A431 cell lysate (the specific EGF concentration and incubation time is confidential information)
Predicted band size : 35 kDa
Observed band size : 35 kDa
- PP2A alpha (phospho Y307) antibody [E155] (ab32104)
![- PP2A alpha (phospho Y307) antibody [E155] (ab32104)](/ps/datasheet/Images/32/ab32104/IHC PP2A alpha (phospho Y307).jpg)
Immunohistochemical analysis of PP2A alpha catalitic subunit (phospho Y307) expression in paraffin-embedded human brain, using 1/50 ab32104.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-PP2A alpha (phospho Y307) antibody [E155](ab32104)
](/ps/datasheet/images/32/ab32104/PP2A-alpha-Primary-antibodies-ab32104-1.jpg)
ab32104 (2µg/ml) staining PP2A alpha (phospho Y307) in human pancreas using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of the islet of Langerhans.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Immunocytochemistry/ Immunofluorescence-PP2A alpha (phospho Y307) antibody [E155](ab32104)
](/ps/datasheet/images/32/ab32104/PP2A-alpha-Primary-antibodies-ab32104-2.jpg)
ICC/IF image of ab32104 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32104, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-PP2A alpha (phospho Y307) antibody [E155] (ab32104)
This product has been referenced in:
- Li T et al. Microcystin-LR (MCLR) Induces a Compensation of PP2A Activity Mediated by a4 Protein in HEK293 Cells. Int J Biol Sci 7:740-52 (2011). WB; Human.Read more (PubMed: 21698000) »
- Kim KY et al. Adiponectin-activated AMPK stimulates dephosphorylation of AKT through protein phosphatase 2A activation. Cancer Res 69:4018-26 (2009). WB; Human.Read more (PubMed: 19366811) »
See all 2 publications for this product
Publishing research using ab32104? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"