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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Overview
Product name
Anti-PPAR alpha antibody
See all PPAR alpha products (11) ...
Description
Rabbit polyclonal to PPAR alpha
Tested applications
IP, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide surrounding amino acid 25 of human PPARa.
Positive control
Rat liver Baboon myometrium single band at ~52 kDa. K562 cells three bands: 52 kDa band and ~60 kDa band have the similar density and a much weaker band at ~45-50 kDa.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, 1% BSA, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Metabolism
Cancer >> Signal transduction >> Nuclear signaling >> Nuclear hormone receptors >> Other
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
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Western blot - PPAR alpha antibody (ab24509)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab24509 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent concentration.
WB: Use a concentration of 3 - 6 µg/ml.Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
Target
Function
Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity). Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2.
Tissue specificity
Skeletal muscle, liver, heart and kidney.
Sequence similarities
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ07869
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- hPPAR antibody
- MGC2237 antibody
- MGC2452 antibody
see all
Database links
- Entrez Gene: 5465 Human
- Entrez Gene: 19013 Mouse
- Entrez Gene: 25747 Rat
- Omim: 170998 Human
- SwissProt: Q07869 Human
- SwissProt: Q6I9S0 Human
- SwissProt: P23204 Mouse
- SwissProt: Q542P9 Mouse
see all
Anti-PPAR alpha antibody images:
Western blot - PPAR alpha antibody (ab24509)
All lanes : Anti-PPAR alpha antibody (ab24509) at 0.5 µg/ml
Lane 1 : Jurkat Cells
Lane 2 : Jurkat Cells
Lane 3 : Rat Liver
Lane 4 : Rat Kidney
Predicted band size : 52 kDa
References for Anti-PPAR alpha antibody (ab24509)
This product has been referenced in:
- Lovren Fet al. Adiponectin primes human monocytes into alternative anti-inflammatory M2 macrophages. Am J Physiol Heart Circ Physiol 299:H656-63 (2010). WB; Human.Read more (PubMed: 20622108) »
- Ji Yet al. Angiotensin II induces inflammatory response partly via toll-like receptor 4-dependent signaling pathway in vascular smooth muscle cells. Cell Physiol Biochem 23:265-76 (2009). WB; Rat.Read more (PubMed: 19471094) »
See all 2 publications for this product
Publishing research using ab24509? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"