Overview
- Product nameAnti-PPAR alpha antibodySee all PPAR alpha primary antibodies ...
- DescriptionRabbit polyclonal to PPAR alpha
- Specificityab61182 detects PPAR alpha protein, (SwissProt Q07869).
- Tested applicationsELISA, ICC/IF, WB more details
- Species reactivityReacts with: Mouse, Hamster, Human
- Immunogen
Synthetic non-phosphopeptide derived from human PPAR alpha around the phosphorylation site of serine 21 (L-E-SP-P-L).
- Positive controlNIH 3T3 cell extracts and HeLa cells.
Properties
- FormLiquid
- Storage instructionsStore at -20°C. Stable for 12 months at -20°C
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
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Research Areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Nucleotide metabolism
- Molecular processes
- Mitochondrial transcription
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Fatty acids
Applications
Our Abpromise guarantee covers the use of ab61182 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | ELISA: 1/5000. |
| ICC/IF | ICC/IF: 1/100 - 1/500. |
| WB | WB: 1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). |
Target
- FunctionLigand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity). Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2.
- Tissue specificitySkeletal muscle, liver, heart and kidney.
- Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. - Cellular localizationNucleus.
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Database links
- Entrez Gene: 5465 Human
- Entrez Gene: 19013 Mouse
- Omim: 170998 Human
- SwissProt: Q6I9S0 Human
- SwissProt: Q07869 Human
- SwissProt: Q542P9 Mouse
- SwissProt: P23204 Mouse
- Unigene: 103110 Human
- Unigene: 710044 Human
- Unigene: 212789 Mouse
see all
Target information above from: UniProt accession
Q07869
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- hPPAR antibodyMGC2237 antibodyMGC2452 antibody
- NR1C1 antibodyNuclear receptor subfamily 1 group C member 1 antibodyOTTHUMP00000197740 antibodyOTTHUMP00000197741 antibodyPeroxisome proliferator activated receptor alpha antibodyPeroxisome proliferator-activated receptor alpha antibodyPPAR antibodyPPAR-alpha antibodyPPARA antibodyPPARA_HUMAN antibodyPPARalpha antibody
see all
Anti-PPAR alpha antibody images
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Immunocytochemistry/ Immunofluorescence - PPAR alpha antibody (ab61182)ab61182 at 1/100 - 1/500 dilution staining PPAR alpha in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide. -
Western blot - PPAR alpha antibody (ab61182)All lanes : Anti-PPAR alpha antibody (ab61182) at 1/500 dilution
Lane 1 : NIH 3T3 cell extracts
Lane 2 : NIH 3T3 cell extracts with the immunising peptide
Predicted band size : 52 kDa
Observed band size : 52 kDa
References for Anti-PPAR alpha antibody (ab61182)
ab61182 has not yet been referenced specifically in any publications.
