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Anti-PPAR alpha (phospho S12) antibody (ab3484)

Abreviews (1)Q&A (3)Specific References (4)

Overview

Applications

Our Abpromise guarantee covers the use of ab3484 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF ICC/IF: Use at an assay dependent concentration.
WB WB: Use a concentration of 2 µg/ml. Predicted molecular weight: 52 kDa.Can be blocked with PPAR alpha peptide (ab4998).

Target

  • FunctionLigand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity). Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2.
  • Tissue specificitySkeletal muscle, liver, heart and kidney.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localizationNucleus.

    • Target information above from: UniProt accession Q07869 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        hPPAR antibodyMGC2237 antibodyMGC2452 antibody
        NR1C1 antibodyNuclear receptor subfamily 1 group C member 1 antibodyOTTHUMP00000197740 antibodyOTTHUMP00000197741 antibodyPeroxisome Proliferator Activated Receptor alpha antibodyPeroxisome proliferator-activated receptor alpha antibodyPPAR antibodyPPAR-alpha antibodyPPARA antibodyPPARA_HUMAN antibodyPPARalpha antibody
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    Anti-PPAR alpha (phospho S12) antibody images

    • Immunocytochemistry/ Immunofluorescence - PPAR alpha (phospho S12) antibody (ab3484)
      Immunocytochemistry/ Immunofluorescence - PPAR alpha (phospho S12) antibody (ab3484)This image is courtesy of an anonymous Abreview
      ab3484 staining PPAR alpha (phospho S12) in Mouse neuronal cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 20 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS) for 18 hours at 4°C. A Cy2®-conjugated Donkey anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.

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    References for Anti-PPAR alpha (phospho S12) antibody (ab3484)

    This product has been referenced in:
    • Qiu L  et al. Inhibition of Aldose Reductase Activates Hepatic Peroxisome Proliferator-Activated Receptor-a and Ameliorates Hepatosteatosis in Diabetic db/db Mice. Exp Diabetes Res 2012:789730 (2012). WB ; Mouse . Read more (PubMed: 22110479) »
    • Jansen S  et al. Glucose deprivation, oxidative stress and peroxisome proliferator-activated receptor-alpha (PPARA) cause peroxisome proliferation in preimplantation mouse embryos. Reproduction 138:493-505 (2009). Read more (PubMed: 19531609) »

    See all 4 Publications for this product

    Publishing research using ab3484 ? Please let us know so that we can cite the reference in this datasheet

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-PPAR alpha (phospho S12) antibody

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    Abreviews
    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (neuronal cells)
    Specification neuronal cells
    Fixative Paraformaldehyde
    Permeabilization No
    Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
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    Submitted Jul 14 2010

    Follow up to previous correspondance: Thank you for your suggestions. I was able to get a signal with all of the antibodies, but I am worried about the sizes of the bands. According to your data sheet, the size of ALL the bands is about 52 kD. However,...

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    Thank you for your recent e-mail. My colleague Jennifer is unexpectedly away this week and I would like to help you. I understand you have recurring problems with ab3484 and ab3485 detecting the wrong bands and your protocol seems fine. I would like t...

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    Thank you for your mail. With the anti-PPARalpha (ab3484) we did not see any signal. With anti-PPARalpha (ab8934) we saw various background bands after 1 h exposure, but not one of the correct size. We loaded 60 micrograms of protein/lane. We did not t...

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    Thanks again for your email. At this point I would like to make the following suggestions that will hopefully help you out. For ab8934 I suggest using 3T3 whole cell lysate as a positive control. For ab3484, I suggest increasing the concentration of the...

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    Thank you for your quick reply. The antibodies which did not function were: ab3484-100 (lot:52477) and ab8934-100 (lot: 44507). I used the antibodies in western blotting, diluted at 1:500 in 5%milk-0.05%Tween-PBS. Secondary antibodies were anti-rabb...

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    Thank you for your email and the details in which you have provided. Can you please tell me - what exactly are you seeing on your blots? Are you not getting any signal at all or are you seeing bands but not at the correct size? How much protein did you ...

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