Anti-PPAR gamma 2 antibody - ChIP Grade (ab45036)
Overview
- Product nameAnti-PPAR gamma 2 antibody - ChIP GradeSee all PPAR gamma 2 primary antibodies ...
- DescriptionRabbit polyclonal to PPAR gamma 2 - ChIP Grade
- SpecificityAb45036 detects peroxisome proliferator activated receptor (PPAR) gamma 2. This antibody does not detect PPAR alpha or PPAR delta.
- Tested applicationsChIP, IP, ICC/IF, WB, Functional Studies more details
- Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Dog, Pig - Immunogen
Synthetic peptide: MGETLGDSPIDPESDSC, corresponding to amino acids 1/16 of Mouse PPAR gamma 2. This sequence is absent in PPAR gamma 1. (Peptide available as ab41762.)
- Positive controlNIH-3T3 cell lysate
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.05% Sodium Azide
Constituents: 1mg/ml BSA -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab45036 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ChIP | ChIP: Use at an assay dependent dilution. PubMed: 21247904 |
| IP | IP: Use at an assay dependent dilution. PubMed: 21247904 |
| ICC/IF | ICC/IF: 1/500. |
| WB | WB: 1/500. Predicted molecular weight: 56 kDa.Can be blocked with PPAR gamma 2 peptide (1-16) (ab41762). By Western blot, this antibody detects an ~56 kDa protein representing PPAR gamma 2 from NIH-3T3 cell lysate. |
| Functional Studies | FuncS: Use at an assay dependent dilution. Ab45036 inhibits PPAR gamma 2 DNA binding. |
Target
- RelevanceThe Peroxisome Proliferator-Activated Receptors (PPARs) are members of the nuclear hormone receptor subfamily of transcription factors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. There are 3 known subtypes of PPARs, PPAR alpha, PPAR delta and PPAR gamma. PPAR gamma is believed to be involved in adipocyte differentiation.
- Cellular localizationNuclear
-
Database links
- Entrez Gene: 281993 Cow
- Entrez Gene: 5468 Human
- Entrez Gene: 19016 Mouse
- Entrez Gene: 397671 Pig
- Entrez Gene: 25664 Rat
- Omim: 601487 Human
- SwissProt: O18971 Cow
- SwissProt: P37231 Human
- SwissProt: P37238 Mouse
- SwissProt: O62807 Pig
- SwissProt: O88275 Rat
- Unigene: 162646 Human
see all
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Alternative names
- NR1C3 antibodyperoxisome proliferative activated receptor gamma isoform 2 antibodyPeroxisome proliferator activated receptor gamma 2 antibody
- PPARG antibodyPPARG2 antibody
see all
Anti-PPAR gamma 2 antibody - ChIP Grade images
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Western blot - Anti-PPAR gamma 2 antibody - ChIP Grade (ab45036)This image is courtesy of Xiaofeng XinLane 1 :
Lane 2 : Anti-PPAR gamma 2 antibody - ChIP Grade (ab45036)
Lane 1 : Protein Marker
Lane 2 : Mouse liver tissue lysate at 1 µg
Secondary
Lane 2 : IRDye® 680RD Donkey anti-Rabbit
Performed under reducing conditions.
Predicted band size : 56 kDa
Exposure time : 2 secondsThis image is courtesy of Xiaofeng Xin
References for Anti-PPAR gamma 2 antibody - ChIP Grade (ab45036)
This product has been referenced in:
- Chen YH et al. Myocyte Enhancer Factor-2 Interacting Transcriptional Repressor (MITR) Is a Switch That Promotes Osteogenesis and Inhibits Adipogenesis of Mesenchymal Stem Cells by Inactivating Peroxisome Proliferator-activated Receptor {gamma}-2. J Biol Chem 286:10671-80 (2011). WB, ChIP, IP ; Human . Read more (PubMed: 21247904) »
