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Anti-PPAR gamma 2 antibody (ab45278)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab45278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).Can be blocked with PPAR gamma 2 peptide (ab45277).

Target

  • RelevanceThe Peroxisome Proliferator-Activated Receptors (PPARs) are members of the nuclear hormone receptor subfamily of transcription factors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. There are 3 known subtypes of PPARs, PPAR alpha, PPAR delta and PPAR gamma. PPAR gamma is believed to be involved in adipocyte differentiation.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
      NR1C3 antibodyperoxisome proliferative activated receptor gamma isoform 2 antibodyPeroxisome proliferator activated receptor gamma 2 antibody
      PPARG antibodyPPARG2 antibody
    see all

Anti-PPAR gamma 2 antibody images

  • Anti-PPAR gamma 2 antibody (ab45278) at 1 µg/ml + Heart (Mouse) Tissue Lysate at 10 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 58 kDa
    Observed band size : 58 kDa

References for Anti-PPAR gamma 2 antibody (ab45278)

ab45278 has not yet been referenced specifically in any publications.

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (heart)
Specification heart
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH9.0
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
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Verified customer

Submitted Oct 30 2008

Application Western blot
Sample Human Cell lysate - whole cell (various incl: CaCo2, SW620, Ms Heart)
Loading amount 30 µg
Specification various incl: CaCo2, SW620, Ms Heart
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Verified customer

Submitted Oct 07 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"