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Synthetic peptide (Human) (C terminal) from a region between AA 420 and 460 of the PPARgamma protein.
Our Abpromise guarantee covers the use of ab19481 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/100. Fix with paraformaldehyde.|
|IHC-P||Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IHC||Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|WB||1/400. Predicted molecular weight: 58 kDa. Block in TBST with 5% w/v nonfat dry milk. The dilution was calculated using an Ecl detection method. With more sensitive detection kits the antibody may be used at much higher dilutions.|
ab19481 staining CHO K1 cells expressing Human PPAR gamma by ICC/IF. Cells were formaldehyde fixed and permeabilized in 0.1 % Triton X-100 prior to blocking with TBS + 3% Dried Milk + 0.1 % Triton X-100 for 20 minutes at 22°C. The primary anitbody was diluted 1/50 and incubated with the sample for 1 hour at 22°C. A FITC conjugated goat anti-rabbit antibody diluted 1/400 was used as the secondary. The cells in the top image clearly show punctate cytoplasmic and nuclear localization of Human PPAR gamma when grown in the presence of F12 + 1.5% Charcoal/Dextran FBS.
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