Overview

  • Product nameAnti-PPAR gamma antibodySee all PPAR gamma primary antibodies ...
  • Description
    Rabbit polyclonal to PPAR gamma
  • Specificitywill recognize both PPAR gamma1 and PPAR gamma2
  • Tested applicationsICC/IF, IHC-P, WB more details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Hamster, Cow, Dog, Human, Pig
  • Immunogen

    Synthetic peptide (Human) (C terminal) from a region between AA 420 and 460 of the PPARgamma protein.

  • Positive control
    • Nuclear lysate of THP-1 cells stimulated with PMA. Incubate the THP-1 cells 10 ng/ml PMA for 24 hours. IHC-P: Human Placenta

Applications

Our Abpromise guarantee covers the use of ab19481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF 1/50 - 1/100. Fix with paraformaldehyde.
IHC-P Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/400. Predicted molecular weight: 58 kDa. Block in TBST with 5% w/v nonfat dry milk. The dilution was calculated using an Ecl detection method. With more sensitive detection kits the antibody may be used at much higher dilutions.

Target

  • FunctionReceptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the receptor binds to a promoter element in the gene for acyl-CoA oxidase and activates its transcription. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis.
  • Tissue specificityHighest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary.
  • Involvement in diseaseNote=Defects in PPARG can lead to type 2 insulin-resistant diabetes and hyptertension. PPARG mutations may be associated with colon cancer.
    Defects in PPARG may be associated with susceptibility to obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
    Defects in PPARG are the cause of familial partial lipodystrophy type 3 (FPLD3) [MIM:604367]. Familial partial lipodystrophies (FPLD) are a heterogeneous group of genetic disorders characterized by marked loss of subcutaneous (sc) fat from the extremities. Affected individuals show an increased preponderance of insulin resistance, diabetes mellitus and dyslipidemia.
    Genetic variations in PPARG can be associated with susceptibility to glioma type 1 (GLM1) [MIM:137800]. Gliomas are central nervous system neoplasms derived from glial cells and comprise astrocytomas, glioblastoma multiforme, oligodendrogliomas, and ependymomas. Note=Polymorphic PPARG alleles have been found to be significantly over-represented among a cohort of American patients with sporadic glioblastoma multiforme suggesting a possible contribution to disease susceptibility.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localizationNucleus.
  • Target information above from: UniProt accession P37231 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • CIMT1 antibody
    • GLM1 antibody
    • HUMPPARG antibody
    • NR1C3 antibody
    • Nuclear receptor subfamily 1 group C member 3 antibody
    • OTTHUMP00000185032 antibody
    • OTTHUMP00000185036 antibody
    • PAX8/PPARG Fusion Gene antibody
    • Peroxisome proliferator activated nuclear receptor gamma variant 1 antibody
    • Peroxisome proliferator activated receptor gamma 1 antibody
    • Peroxisome Proliferator Activated Receptor gamma antibody
    • Peroxisome proliferator-activated receptor gamma antibody
    • PPAR gamma antibody
    • PPAR-gamma antibody
    • PPARG antibody
    • PPARG_HUMAN antibody
    • PPARG1 antibody
    • PPARG2 antibody
    • PPARG3 antibody
    see all

Anti-PPAR gamma antibody images

  • ab19481 staining PPARγ in U-87 MG cells treated with ciglitazone (ab141139), by ICC/IF. Increase of PPARγ cytoplasmic expression correlates with increased concentration of ciglitazone, as described in literature.
    The cells were incubated at 37°C for 24 hours in media containing different concentrations of ab141139 (ciglitazone) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab19481 (5 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  • ab19481 staining CHO K1 cells expressing Human PPAR gamma by ICC/IF.  Cells were formaldehyde fixed and permeabilized in 0.1 % Triton X-100 prior to blocking with TBS + 3% Dried Milk + 0.1 % Triton X-100 for 20 minutes at 22°C.  The primary anitbody was diluted 1/50 and incubated with the sample for 1 hour at 22°C.  A FITC conjugated goat anti-rabbit antibody diluted 1/400 was used as the secondary.  The cells in the top image clearly show punctate cytoplasmic and nuclear localization of Human PPAR gamma when grown in the presence of F12 + 1.5% Charcoal/Dextran FBS.

    See Abreview

  • Ab19481 staining Human normal placenta. Staining is localized to nuclear compartment.
    Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • ICC/IF image of ab19481 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19481, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-PPAR gamma antibody (ab19481)

This product has been referenced in:
  • Knapp P  et al. Altered peroxisome-proliferator activated receptors expression in human endometrial cancer. PPAR Res 2012:471524 (2012). WB ; Human . Read more (PubMed: 22448166) »
  • Donzelli E  et al. ERK1 and ERK2 are involved in recruitment and maturation of human mesenchymal stem cells induced to adipogenic differentiation. J Mol Cell Biol 3:123-31 (2011). WB ; Human . Read more (PubMed: 21278199) »

See all 7 Publications for this product

Product Wall

Thank you for your reply.
Yes, that is indeed strange. Unfortunately, I do not have a good explanation as to what could cause the antibody bind to the protein in this streaky way.
I was going to offer you a free of charge replacement as compe...

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Thank you for submitting an Abreview of ab19481. As you may have noticed, your review has now been published on our website.
Since you obtained poor results using the antibody in a tested species and application, we would like to follow up on this...

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Application Western blot
Sample Rat Tissue lysate - nuclear (Rat liver tissue)
Loading amount 50 µg
Specification Rat liver tissue
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mrs. Smitha Koshy

Verified customer

Submitted Jul 25 2012

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has c...

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Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.
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The details provided will enable us to investigate this case and will provide us with vital information fo...

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Thank you for your response.

Each antibody has specific storage buffer and recommended storage conditions. I would suggest checking this information carefully and follow the instructions. Generally, we suggest customers to use our antibodies ...

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Thank you for providing some further details of the cells/samples you wish to use in the experiments.

ab59256 and ab19481 both have been tested for ICC/IF and they detect human protein so they would suit your need. However, since the cell typ...

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Thank you for your enquiry.

We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storag...

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Thank you for confirming these details. I appreciate the time taken to submit further information to us and I would like to make some suggestions to the protocol in an attempt to improve the results obtained: 1) To completely denature the protein...

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Thank you for taking the time to contact us. I am sorry to hear your customer has had difficulty obtaining satisfactory results from this PPAR gamma antibody. The details you have kindly provided will enable us to investigate this case for you and a...

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