Overview
- Product nameAnti-PPAR gamma antibodySee all PPAR gamma primary antibodies ...
- DescriptionRabbit polyclonal to PPAR gamma
- Tested applicationsWB, IHC-FoFr more details
- Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Rabbit - Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human PPAR gamma .
(Peptide available as ab32241.)
- Positive controlThis antibody gave a positive signal in the following tissue lysates: Mouse Brown Adipose Tissue Lysate Mouse Heart Mouse Testis Mouse Ovary Rat Heart
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab27649 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).Can be blocked with PPAR gamma peptide (ab32241). This is the predicted weight of isoform 1 of PPAR gamma as described in Swissprot entry P37238. |
| IHC-FoFr | IHC-FoFr: Use at an assay dependent dilution. PubMed: 20664693 |
Target
- FunctionReceptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the receptor binds to a promoter element in the gene for acyl-CoA oxidase and activates its transcription. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis.
- Tissue specificityHighest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary.
- Involvement in diseaseNote=Defects in PPARG can lead to type 2 insulin-resistant diabetes and hyptertension. PPARG mutations may be associated with colon cancer.
Defects in PPARG may be associated with susceptibility to obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
Defects in PPARG are the cause of familial partial lipodystrophy type 3 (FPLD3) [MIM:604367]. Familial partial lipodystrophies (FPLD) are a heterogeneous group of genetic disorders characterized by marked loss of subcutaneous (sc) fat from the extremities. Affected individuals show an increased preponderance of insulin resistance, diabetes mellitus and dyslipidemia.
Genetic variations in PPARG can be associated with susceptibility to glioma type 1 (GLM1) [MIM:137800]. Gliomas are central nervous system neoplasms derived from glial cells and comprise astrocytomas, glioblastoma multiforme, oligodendrogliomas, and ependymomas. Note=Polymorphic PPARG alleles have been found to be significantly over-represented among a cohort of American patients with sporadic glioblastoma multiforme suggesting a possible contribution to disease susceptibility. - Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. - Cellular localizationNucleus.
-
Database links
- Entrez Gene: 5468 Human
- Entrez Gene: 5468 Human
- Entrez Gene: 19016 Mouse
- Entrez Gene: 25664 Rat
- Omim: 601487 Human
- SwissProt: P37231 Human
- SwissProt: P37231 Human
- SwissProt: P37238 Mouse
- SwissProt: O19052 Rabbit
- SwissProt: O88275 Rat
- Unigene: 162646 Human
- Unigene: 3020 Mouse
- Unigene: 23443 Rat
see all
Target information above from: UniProt accession
P37231
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
-
Alternative names
- CIMT1 antibodyGLM1 antibodyHUMPPARG antibody
- NR1C3 antibodyNuclear receptor subfamily 1 group C member 3 antibodyOTTHUMP00000185032 antibodyOTTHUMP00000185036 antibodyPAX8/PPARG Fusion Gene antibodyPAX8/PPARG Fusion Gene antibodyPeroxisome proliferator activated nuclear receptor gamma variant 1 antibodyPeroxisome proliferator activated receptor gamma 1 antibodyPeroxisome Proliferator Activated Receptor gamma antibodyPeroxisome Proliferator Activated Receptor gamma antibodyPeroxisome proliferator-activated receptor gamma antibodyPPAR gamma antibodyPPAR-gamma antibodyPPARG antibodyPPARG_HUMAN antibodyPPARG1 antibodyPPARG1 antibodyPPARG2 antibodyPPARG2 antibodyPPARG3 antibodyPPARG3 antibody
see all
Anti-PPAR gamma antibody images
-
Western blot - PPAR gamma antibody (ab27649)Anti-PPAR gamma antibody (ab27649) at 1 µg/ml + Mouse Brown Adipose Tissue Lysate at 20 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 54 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
Additional bands at : 125 kDa (possible non-specific binding). -
Western blot - PPAR gamma antibody (ab27649)All lanes : Anti-PPAR gamma antibody (ab27649) at 1 µg/ml
Lane 1 : Heart (Mouse) Tissue Lysate
Lane 2 : Testis (Mouse) Tissue Lysate
Lane 3 : Ovary (Mouse) Tissue Lysate - normal tissue
Lane 4 : Heart (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 54 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.
References for Anti-PPAR gamma antibody (ab27649)
This product has been referenced in:
- Tiyerili V et al. Estrogen improves vascular function via peroxisome-proliferator-activated-receptor-?. J Mol Cell Cardiol 53:268-76 (2012). WB ; Mouse . Read more (PubMed: 22634137) »
- Babaev VR et al. Macrophage Mal1 deficiency suppresses atherosclerosis in low-density lipoprotein receptor-null mice by activating peroxisome proliferator-activated receptor-?-regulated genes. Arterioscler Thromb Vasc Biol 31:1283-90 (2011). WB ; Mouse . Read more (PubMed: 21474828) »
