Overview

  • Product nameAnti-PPAR gamma antibody
    See all PPAR gamma primary antibodies
  • Description
    Rabbit polyclonal to PPAR gamma
  • Tested applicationsWB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human PPAR gamma .

    (Peptide available as ab32241.)

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Mouse Brown Adipose Tissue Lysate Mouse Heart Mouse Testis Mouse Ovary Rat Heart

Properties

Applications

Our Abpromise guarantee covers the use of ab27649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).Can be blocked with Human PPAR gamma peptide (ab32241). This is the predicted weight of isoform 1 of PPAR gamma as described in Swissprot entry P37238.
IHC-FoFr Use at an assay dependent dilution. PubMed: 20664693

Target

  • FunctionReceptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the receptor binds to a promoter element in the gene for acyl-CoA oxidase and activates its transcription. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis.
  • Tissue specificityHighest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary.
  • Involvement in diseaseNote=Defects in PPARG can lead to type 2 insulin-resistant diabetes and hyptertension. PPARG mutations may be associated with colon cancer.
    Defects in PPARG may be associated with susceptibility to obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
    Defects in PPARG are the cause of familial partial lipodystrophy type 3 (FPLD3) [MIM:604367]. Familial partial lipodystrophies (FPLD) are a heterogeneous group of genetic disorders characterized by marked loss of subcutaneous (sc) fat from the extremities. Affected individuals show an increased preponderance of insulin resistance, diabetes mellitus and dyslipidemia.
    Genetic variations in PPARG can be associated with susceptibility to glioma type 1 (GLM1) [MIM:137800]. Gliomas are central nervous system neoplasms derived from glial cells and comprise astrocytomas, glioblastoma multiforme, oligodendrogliomas, and ependymomas. Note=Polymorphic PPARG alleles have been found to be significantly over-represented among a cohort of American patients with sporadic glioblastoma multiforme suggesting a possible contribution to disease susceptibility.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CIMT1 antibody
    • GLM1 antibody
    • HUMPPARG antibody
    • NR1C3 antibody
    • Nuclear receptor subfamily 1 group C member 3 antibody
    • OTTHUMP00000185032 antibody
    • OTTHUMP00000185036 antibody
    • PAX8/PPARG Fusion Gene antibody
    • PAX8/PPARG Fusion Gene antibody
    • Peroxisome proliferator activated nuclear receptor gamma variant 1 antibody
    • Peroxisome proliferator activated receptor gamma 1 antibody
    • Peroxisome Proliferator Activated Receptor gamma antibody
    • Peroxisome Proliferator Activated Receptor gamma antibody
    • Peroxisome proliferator-activated receptor gamma antibody
    • PPAR gamma antibody
    • PPAR-gamma antibody
    • PPARG antibody
    • PPARG_HUMAN antibody
    • PPARG1 antibody
    • PPARG1 antibody
    • PPARG2 antibody
    • PPARG2 antibody
    • PPARG3 antibody
    • PPARG3 antibody
    • PPARgamma antibody
    see all

Anti-PPAR gamma antibody images

  • Anti-PPAR gamma antibody (ab27649) at 1 µg/ml + Mouse Brown Adipose Tissue Lysate at 20 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 54 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 125 kDa (possible non-specific binding).
  • All lanes : Anti-PPAR gamma antibody (ab27649) at 1 µg/ml

    Lane 1 : Heart (Mouse) Tissue Lysate
    Lane 2 : Testis (Mouse) Tissue Lysate
    Lane 3 : Ovary (Mouse) Tissue Lysate - normal tissue
    Lane 4 : Heart (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 54 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.

References for Anti-PPAR gamma antibody (ab27649)

This product has been referenced in:
  • Wang Y  et al. Comparison between the efficacies of curcumin and puerarin in C57BL/6 mice with steatohepatitis induced by a methionine- and choline-deficient diet. Exp Ther Med 7:663-668 (2014). WB ; Mouse . Read more (PubMed: 24520264) »
  • Zhang Y  et al. Regulation of lipid and glucose homeostasis by mango tree leaf extract is mediated by AMPK and PI3K/AKT signaling pathways. Food Chem 141:2896-905 (2013). WB ; Mouse . Read more (PubMed: 23871039) »

See all 8 Publications for this product

Product Wall

Thank you for your reply.

The Anti-PPAR gamma antibody (ab27649) is provided already reconstituted in liquid form in a PBS buffer (pH 7.4) with 1% BSA and 0.02% sodium azide supplemented. The customer therefore does not need to reconstitute i...

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Thank you for contacting us.

I am assuming customer is interested in molecular weight of proteins these antibodies detect. The predicted band size is as follows;

ab28481; the molecular weight of bands would be 121, 113, 111 and 124 kD...

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Thank you very much for your interest in ab27649. To our knowledge, ab27649 has not been tested in rabbit. The crossreactivity with rabbit has been predicted based on sequence homology. However, we offer testing discounts for trying an antibody in unte...

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Thank you for contacting us. Having looked at the antibodies available for PPAR gamma for rat and WB, ab19481, ab27649 and ab70405 have all been tested in this application and species. ab19481 has also been tested with rat samples and as you sa...

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Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain Cortex)
Specification Brain Cortex
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

Submitted May 21 2010

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Placenta)
Specification Placenta
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval Solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Apr 20 2010

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (IB3-1 cystic fibrosis bronchial epithelial cell li)
Specification IB3-1 cystic fibrosis bronchial epithelial cell li
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100 in PBS
Username

Abcam user community

Verified customer

Submitted Sep 16 2009

Thank you for your enquiry. I have conducted a sequence alignment of the immunogen for ab27649 PPAR gamma antibody with PPAR gamma 1 and PPAR gamma 2. I can confirm the sequence has 100% alignment with both, so will detect both isoforms. I hope ...

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Thank you for contacting us for technical support on behalf of your customer. I would like to suggest the following modifications to the protocol: 1) Major modifications: -use reducing conditions (reducing gel, reducing loading buffer) -use the...

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