Overview
- Product nameAnti-PPHLN1 antibodySee all PPHLN1 primary antibodies ...
- DescriptionRabbit polyclonal to PPHLN1
- Specificityab69569 detects endogenous levels of total PPHLN protein.
- Tested applicationsICC/IF, WB, ELISA, IHC-P more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse - Immunogen
Synthesized peptide derived from an internal region of human PPHLN1.
- Positive controlExtracts from COLO or 293 cells. Liver carcinoma tissue.
Properties
- FormLiquid
- Storage instructionsStore at -20°C. Stable for 12 months at -20°C
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab69569 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | ICC/IF: Use at an assay dependent concentration. |
| WB | WB: 1/500 - 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa). |
| ELISA | ELISA: 1/20000. |
| IHC-P | IHC-P: 1/50 - 1/100. |
Target
- FunctionInvolved in epithelial differentiation and contributes to epidermal integrity and barrier formation.
- Tissue specificityUbiquitous.
- Post-translational
modificationsSubstrate of transglutaminase (in vitro). - Cellular localizationNucleus. Cytoplasm. In undifferentiated keratinocytes expressed in speckle-type nuclear granules and at the nuclear membrane, but in the differentiated keratinocytes colocalized with periplakin at the cell periphery and at cell-cell junctions.
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Database links
- Entrez Gene: 51535 Human
- Entrez Gene: 223828 Mouse
- Omim: 608150 Human
- SwissProt: Q8NEY8 Human
- SwissProt: Q8K2H1 Mouse
- Unigene: 444157 Human
- Unigene: 74208 Mouse
Target information above from: UniProt accession
Q8NEY8
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Gastric cancer antigen Ga50 antibodyPeriphilin 1 antibodyPeriphilin-1 antibody
- PPHLN_HUMAN antibodyPPHLN1 antibody
see all
Anti-PPHLN1 antibody images
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Western blot - PPHLN1 antibody (ab69569)All lanes : Anti-PPHLN1 antibody (ab69569) at 1/500 dilution
Lane 1 : Extracts from COLO cells
Lane 2 : Extracts from 293 cells
Lane 3 : Extracts from COLO cells with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 53 kDa
Observed band size : 53 kDa
Additional bands at : 100 kDa,35 kDa,40 kDa,55 kDa,85 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PPHLN1 antibody (ab69569)Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using 1/50-1/100 ab69569. For the image in the right panel, the antibody was preincubated with the immunizing peptide.
References for Anti-PPHLN1 antibody (ab69569)
This product has been referenced in:
- Martrat G et al. Exploring the link between MORF4L1 and risk of breast cancer. Breast Cancer Res 13:R40 (2011). Read more (PubMed: 21466675) »
