The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
1/500 - 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
1/50 - 1/100.
Involved in epithelial differentiation and contributes to epidermal integrity and barrier formation.
Substrate of transglutaminase (in vitro).
Nucleus. Cytoplasm. In undifferentiated keratinocytes expressed in speckle-type nuclear granules and at the nuclear membrane, but in the differentiated keratinocytes colocalized with periplakin at the cell periphery and at cell-cell junctions.