Overview

  • Product nameAnti-PPHLN1 antibody
    See all PPHLN1 primary antibodies
  • Description
    Rabbit polyclonal to PPHLN1
  • Specificityab69569 detects endogenous levels of total PPHLN protein.
  • Tested applicationsICC/IF, WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthesized peptide derived from an internal region of human PPHLN1.

  • Positive control
    • Extracts from COLO or 293 cells. Liver carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab69569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
ELISA 1/20000.
IHC-P 1/50 - 1/100.

Target

  • FunctionInvolved in epithelial differentiation and contributes to epidermal integrity and barrier formation.
  • Tissue specificityUbiquitous.
  • Post-translational
    modifications
    Substrate of transglutaminase (in vitro).
  • Cellular localizationNucleus. Cytoplasm. In undifferentiated keratinocytes expressed in speckle-type nuclear granules and at the nuclear membrane, but in the differentiated keratinocytes colocalized with periplakin at the cell periphery and at cell-cell junctions.
  • Information by UniProt
  • Database links
  • Alternative names
    • Gastric cancer antigen Ga50 antibody
    • Periphilin 1 antibody
    • Periphilin-1 antibody
    • PPHLN_HUMAN antibody
    • PPHLN1 antibody
    see all

Anti-PPHLN1 antibody images

  • All lanes : Anti-PPHLN1 antibody (ab69569) at 1/500 dilution

    Lane 1 : Extracts from COLO cells
    Lane 2 : Extracts from 293 cells
    Lane 3 : Extracts from COLO cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 53 kDa
    Observed band size : 53 kDa
    Additional bands at : 100 kDa,35 kDa,40 kDa,55 kDa,85 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using 1/50-1/100 ab69569. For the image in the right panel, the antibody was preincubated with the immunizing peptide.

References for Anti-PPHLN1 antibody (ab69569)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Formaldehyde
Permeabilization Yes - 0.5% Triton X-100
Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 20°C
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Verified customer

Submitted Nov 01 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 40 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing (8%)
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 20°C
Username

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Verified customer

Submitted Oct 31 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"