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RabMAbs (1950-1)

Anti-PPP1CA + 1CB [EP1511Y] antibody (ab52619)

Submit an Abreview Q&A (1)Specific References (2)

Overview

  • Product nameAnti-PPP1CA + 1CB [EP1511Y] antibody
  • Description
    Rabbit monoclonal [EP1511Y] to PPP1CA + 1CB
  • SpecificityThe immunogen for ab52619 is 100% homologous with Human PPP1CA and PPP1CB
  • Tested applicationsWB, IP, ICC, IHC-P, Flow Cyt more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues on human PP1 alpha.

  • Positive controlHeLa cell lysate, human uterus carcinoma tissue. This antibody gave a positive result in IF/ICC when used in the following formaldehyde/methanol fixed cell lines: DU145.
  • General notesProduced under U.S. Patent No. 5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab52619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/200000. Predicted molecular weight: 37 kDa.
IP IP: 1/100.
ICC ICC: 1/100 - 1/250.
IHC-P IHC-P: Use at an assay dependent dilution.
Flow Cyt Flow Cyt: 1/100.

Target

  • Alternative names
      Alpha isoform serine threonine protein phosphatase PP1alpha 1 catalytic subunit antibodyCatalytic subunit antibodyEC 3.1.3.16 antibody
      MGC15877 antibodyMGC1674 antibodyMGC9201 antibodyPP 1A antibodyPP-1A antibodyPP1A antibodyPP1A_HUMAN antibodyPP1alpha antibodyPP2C ALPHA antibodyPP2CA antibodyPPP1CA antibodyProtein Phosphatase 2C Alpha Isoform antibodySerine threonine protein phosphatase PP1 alpha catalytic subunit antibodySerine threonine protein phosphatase PP1 alpha catalytic subunit protein phosphatase 1 antibodySerine/threonine-protein phosphatase PP1-alpha catalytic subunit antibody
    see all

Anti-PPP1CA + 1CB [EP1511Y] antibody images

  • Western blot - Anti-PPP1CA + 1CB [EP1511Y] antibody (ab52619)
    Western blot - Anti-PPP1CA + 1CB [EP1511Y] antibody (ab52619)
    Anti-PPP1CA + 1CB [EP1511Y] antibody (ab52619) at 1/200000 dilution + HeLa cell lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP antibody at 1/2000 dilution

    Predicted band size : 37 kDa
    Observed band size : 37 kDa
  • Anti-PPP1CA + 1CB [EP1511Y] antibody (ab52619)
    Anti-PPP1CA + 1CB [EP1511Y] antibody (ab52619)
    Ab52619 at 1/100 dilution staining human uterus carcinoma tissue; paraffin embedded.Ab52619 at 1/100 dilution staining human uterus carcinoma tissue; paraffin embedded.
  • Flow Cytometry - Anti-PPP1CA + 1CB [EP1511Y] antibody (ab52619)
    Flow Cytometry - Anti-PPP1CA + 1CB [EP1511Y] antibody (ab52619)
    Overlay histogram showing HeLa cells stained with ab52619 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52619, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Immunocytochemistry/ Immunofluorescence - Anti-PPP1CA + 1CB [EP1511Y] antibody (ab52619)
    Immunocytochemistry/ Immunofluorescence - Anti-PPP1CA + 1CB [EP1511Y] antibody (ab52619)

    ICC/IF image of ab52619 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab52619 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-PPP1CA + 1CB [EP1511Y] antibody (ab52619)

This product has been referenced in:
  • Horiuchi D  et al. MYC pathway activation in triple-negative breast cancer is synthetic lethal with CDK inhibition. J Exp Med 209:679-96 (2012). Read more (PubMed: 22430491) »
  • Kuehnen P  et al. Protein phosphatase 1 (PP-1)-dependent inhibition of insulin secretion by leptin in INS-1 pancreatic ß-cells and human pancreatic islets. Endocrinology 152:1800-8 (2011). Read more (PubMed: 21427225) »

See all 2 Publications for this product

Publishing research using ab52619 ? Please let us know so that we can cite the reference in this datasheet

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Will these antibodies cross react with other isoforms of the protein.

Thank you for calling Abcam earlier today.

I blasted the epiopte sequence for ab52619 and I do not think it would recognize other isoforms. But below is the immunogen sequence, if you would like to double check:
*



If there ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"