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Overview

  • Product namePPP1R7 protein (Tagged-His Tag)
  • Protein descriptionRecombinant full length protein, corresponding to amino acids 1-360 of Human PPP1R7 isoform 1 with N terminal His tag, 360 amino acids, 55kDa. UniProt ID = Q15435.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 135 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch).
  • Sequence notesMAAERGAGQQQSQEMMEVDRRVESEESGDEEGKKHSSGIV ADL SEQSLKDGEERGEEDPEEEHELPVDMETINLDRDA EDVDLNHY RIGKIEGFEVLKKVKTLCLRQNLIKCIENL EELQSLRELDLYD NQIKKIENLEALTELEILDISFNLL RNIEGVDKLTRLKKLFLV NNKISKIENLSNLHQLQMLE LGSNRIRAIENIDTLTNLESLFL GKNKITKLQNLDALT NLTVLSMQSNRLTKIEGLQNLVNLRELY LSHNGIEVIE GLENNNKLTMLDIASNRIKKIENISHLTELQEF WMNDN LLESWSDLDELKGARSLETVYLERNPLQKDPQYRRKVM LALPSVRQIDATFVRF
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91712 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        MGC105784PP1R7_HUMANPPP1R7
        protein phosphatase 1 regulatory (inhibitor) subunit 7Protein phosphatase 1 regulatory subunit 22Protein phosphatase 1 regulatory subunit 7Protein phosphatase 1 regulatory subunit 7 alpha2Protein phosphatase 1 regulatory subunit 7 beta1Protein phosphatase 1 regulatory subunit 7 beta2SDS 22SDS22
      see all
  • FunctionRegulatory subunit of protein phosphatase 1.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the SDS22 family.
    Contains 11 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
  • Cellular localizationNucleus.

    • Target information above from: UniProt accession Q15435 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    PPP1R7 protein (Tagged-His Tag) images

    • SDS-PAGE - PPP1R7 protein (Tagged-His Tag) (ab91712)
      SDS-PAGE - PPP1R7 protein (Tagged-His Tag) (ab91712)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for PPP1R7 protein (Tagged-His Tag) (ab91712)

    ab91712 has not yet been referenced specifically in any publications.

    Publishing research using ab91712 ? Please let us know so that we can cite the reference in this datasheet

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