Recombinant Anti-PPP2R2D antibody [EPR13624] (ab181071)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13624] to PPP2R2D
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-PPP2R2D antibody [EPR13624]
See all PPP2R2D primary antibodies -
Description
Rabbit monoclonal [EPR13624] to PPP2R2D -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, SH-SY5Y and Human fetal brain lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13624 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/2000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
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Notes |
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WB
1/1000 - 1/2000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). |
Target
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Function
B regulatory subunit of protein phosphatase 2A (PP2A) that plays a key role in cell cycle by controlling mitosis entry and exit. The activity of PP2A complexes containing PPP2R2D (PR55-delta) fluctuate during the cell cycle: the activity is high in interphase and low in mitosis. During mitosis, activity of PP2A is inhibited via interaction with phosphorylated ENSA and ARPP19 inhibitors. Within the PP2A complexes, the B regulatory subunits modulate substrate selectivity and catalytic activity, and also may direct the localization of the catalytic enzyme to a particular subcellular compartment. -
Sequence similarities
Belongs to the phosphatase 2A regulatory subunit B family.
Contains 7 WD repeats. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 55844 Human
- Entrez Gene: 52432 Mouse
- Entrez Gene: 246255 Rat
- Omim: 613992 Human
- SwissProt: Q66LE6 Human
- SwissProt: Q925E7 Mouse
- SwissProt: P56932 Rat
- Unigene: 380372 Human
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Alternative names
- 2ABD_HUMAN antibody
- MDS026 antibody
- PP2A subunit B isoform B55-delta antibody
see all
Images
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Anti-PPP2R2D antibody [EPR13624] (ab181071) at 1/5000 dilution (purified) + Rat brain tissue lysate at 20 µg
Secondary
HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDaBlocking Buffer: 5% NFDM/TBST
Dilution Buffer: 5% NFDM/TBST
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Anti-PPP2R2D antibody [EPR13624] (ab181071) at 1/5000 dilution (purified) + Mouse brain tissue lysate
Secondary
HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDaBlocking Buffer: 5% NFDM/TBST
Dilution Buffer: 5% NFDM/TBST
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All lanes : Anti-PPP2R2D antibody [EPR13624] (ab181071) at 1/1000 dilution (purified)
Lane 1 : SH-SY5Y cell lysate
Lane 2 : Human fetal brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDaBlocking Buffer: 5% NFDM/TBST
Dilution Buffer: 5% NFDM/TBST
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All lanes : Anti-PPP2R2D antibody [EPR13624] (ab181071) at 1/2000 dilution (unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : Human fetal brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab181071 has been referenced in 2 publications.
- Pan W et al. PPP2R2D suppresses IL-2 production and Treg function. JCI Insight 5:N/A (2020). PubMed: 32897879
- Yu S et al. PPP2R2D, a regulatory subunit of protein phosphatase 2A, promotes gastric cancer growth and metastasis via mechanistic target of rapamycin activation. Int J Oncol 52:2011-2020 (2018). PubMed: 29568966