Anti-PPT1 antibody - N-terminal (ab135516)

Overview

  • Product nameAnti-PPT1 antibody - N-terminalSee all PPT1 primary antibodies ...
  • Description
    Rabbit polyclonal to PPT1 - N-terminal
  • Tested applicationsWB, IHC-P more details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 40-69 of Human PPT1

  • Positive control
    • Mouse cerebellum tissue lysate.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesab135516 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab135516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/100 - 1/500. Predicted molecular weight: 34 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionRemoves thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons.
  • Involvement in diseaseDefects in PPT1 are the cause of neuronal ceroid lipofuscinosis type 1 (CLN1) [MIM:256730]. A form of neuronal ceroid lipofuscinosis with variable age at onset. Infantile, late-infantile, juvenile, and adult onset have been reported. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy. The lipopigment pattern seen most often in CLN1 is referred to as granular osmiophilic deposits (GROD).
  • Sequence similaritiesBelongs to the palmitoyl-protein thioesterase family.
  • Cellular localizationLysosome.
  • Target information above from: UniProt accession P50897 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Ceroid palmitoyl palmitoyl protein thioesterase 1 antibody
    • CLN1 antibody
    • EC 3.1.2.22 antibody
    • INCL antibody
    • Palmitoyl protein hydrolase 1 antibody
    • Palmitoyl protein thioesterase 1 antibody
    • Palmitoyl-protein hydrolase 1 antibody
    • Palmitoyl-protein thioesterase 1 antibody
    • PPT antibody
    • PPT-1 antibody
    • PPT1 antibody
    • PPT1_HUMAN antibody
    see all

Anti-PPT1 antibody - N-terminal images

  • Anti-PPT1 antibody - N-terminal (ab135516) at 1/100 dilution + Mouse cerebellum tissue lysate. at 35 µg
    developed using the ECL technique

    Predicted band size : 34 kDa

References for Anti-PPT1 antibody - N-terminal (ab135516)

ab135516 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Sample Mouse Cell (over expression of mPPT1 in Cos7 cells)
Specification over expression of mPPT1 in Cos7 cells
Permeabilization Yes - 0.25% Tx-100 or 50ug.ml of digitonin
Fixative Paraformaldehyde
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Submitted May 06 2013

Application Western blot
Sample Mouse Cell lysate - whole cell (L cells (mouse fibroblast cells))
Loading amount 15000 cells
Specification L cells (mouse fibroblast cells)
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Submitted Apr 29 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"