Anti-PPTC7 antibody (ab122548)
Key features and details
- Rabbit polyclonal to PPTC7
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PPTC7 antibody -
Description
Rabbit polyclonal to PPTC7 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
amino acids 118-215 (ILTTSYCELL QNKVPLLGSS TACIVVLDRT SHRLHTANLG DSGFLVVRGG EVVHRSDEQQ HYFNTPFQLS IAPPEAEGVV LSDSPDAADS TSFDVQL) of the Human PPTC7 protein (Q8NI37).
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Positive control
- IHC-P: Human testis, rectum, liver and kidney tissue. WB: RT-4, U-251 MG, NIH-3T3, NBT-II. ICC/IF: A-431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122548 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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Notes |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
Target
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Sequence similarities
Belongs to the PP2C family.
Contains 1 PP2C-like domain. - Information by UniProt
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Database links
- Entrez Gene: 160760 Human
- Entrez Gene: 320717 Mouse
- Entrez Gene: 304488 Rat
- Omim: 609668 Human
- SwissProt: Q8NI37 Human
- SwissProt: Q6NVE9 Mouse
- Unigene: 13854 Human
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Alternative names
- pptc7 antibody
- PPTC7_HUMAN antibody
- Protein phosphatase PTC7 homolog antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human testis tissue labeling PPTC7 with ab122548 at a 1/500 dilution.
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All lanes : Anti-PPTC7 antibody (ab122548) at 0.4 µg/ml
Lane 1 : RT4 Cell lysate
Lane 2 : U 251 MG Cell lysate -
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli, plasma membrane and cytoplasm. Recommended concentration of ab122548 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human rectum tissue labeling PPTC7 with ab122548 at a 1/500 dilution.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human kidney tissue labeling PPTC7 with ab122548 at a 1/500 dilution.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human liver tissue labeling PPTC7 with ab122548 at a 1/500 dilution.
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All lanes : Anti-PPTC7 antibody (ab122548) at 0.4 µg/ml
Lane 1 : NIH-3T3 cell lysate
Lane 2 : NBT-II cell lysate
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab122548 has been referenced in 1 publication.
- Mishra A et al. A protein phosphatase network controls the temporal and spatial dynamics of differentiation commitment in human epidermis. Elife 6:N/A (2017). PubMed: 29043977