Anti-PQBP1 antibody (ab100797)
Key features and details
- Rabbit polyclonal to PQBP1
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PQBP1 antibody -
Description
Rabbit polyclonal to PQBP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Horse, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide within Human PQBP1 aa 150-250. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_005701.1
Database link: O60828 -
Positive control
- HeLa, 293T lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to PQBP1 immobilized on solid support -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab100797 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 30 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 30 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
May suppress the ability of POU3F2 to transactivate the DRD1 gene in a POU3F2 dependent manner. Can activate transcription directly or via association with the transcription machinery. May be involved in ATXN1 mutant-induced cell death. The interaction with ATXN1 mutant reduces levels of phosphorylated RNA polymerase II large subunit. -
Tissue specificity
Widely expressed with high level in heart, skeletal muscle, pancreas, spleen, thymus, prostate, ovary, small intestine and peripheral blood leukocytes. -
Involvement in disease
Defects in PQBP1 are the cause of Renpenning syndrome 1 (RENS1) [MIM:309500]; also known as Sutherland-Haan X-linked mental retardation syndrome (SHS) or X-linked mental retardation syndromes MRXS3/MRXS8/MRX55. The clinical features are mental retardation, microcephaly, short stature, and small testes. The craniofacies tends to be narrow and tall with upslanting palpebral fissures, abnormal nasal configuration, cupped ears, and short philtrum. The nose may appear long or bulbous, with overhanging columella. Less consistent manifestations include ocular colobomas, cardiac malformations, cleft palate, and anal anomalies. RENS1 is more frequently in males than in females where little or no expression is found. -
Sequence similarities
Contains 1 WW domain. -
Domain
The WW domain may play a role as a transcriptional activator directly or via association with the transcription machinery. The WW domain mediates interaction with C-terminal domain of RNA polymerase II large subunit. -
Cellular localization
Nucleus. Co-localized with POU3F2. Co-localized with ATXN1 in nuclear inclusion bodies. - Information by UniProt
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Database links
- Entrez Gene: 10084 Human
- Entrez Gene: 54633 Mouse
- Omim: 300463 Human
- SwissProt: O60828 Human
- SwissProt: Q91VJ5 Mouse
- Unigene: 534384 Human
- Unigene: 14616 Mouse
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Alternative names
- 38 kDa nuclear protein containing a WW domain antibody
- Mental retardation, X linked 55 antibody
- MRX55 antibody
see all
Images
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All lanes : Anti-PQBP1 antibody (ab100797) at 0.1 µg/ml
Lane 1 : HeLa whole Cell Lysate at 50 µg
Lane 2 : HeLa whole Cell Lysate at 15 µg
Lane 3 : HeLa whole Cell Lysate at 5 µg
Lane 4 : 293T whole Cell Lysate at 50 µg
Predicted band size: 30 kDa
Exposure time: 30 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling PQBP1 with ab100797 at 1/1000 (1µg/mg). Detection: DAB.
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ab100797 at 1 µg/ml staining PQBP1 in HeLa cell lysate immunoprecipitated using ab100797 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Exposure time: 10 seconds
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab100797 has been referenced in 1 publication.
- Yang H et al. CPVL promotes glioma progression via STAT1 pathway inhibition through interactions with the BTK/p300 axis. JCI Insight 6:N/A (2021). PubMed: 34784299