Recombinant Anti-PRA1 antibody [EPR1747Y] (ab76413)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1747Y] to PRA1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PRA1 antibody [EPR1747Y]
See all PRA1 primary antibodies -
Description
Rabbit monoclonal [EPR1747Y] to PRA1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human PRA1 (C terminal). The exact sequence is proprietary.
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Positive control
- WB: HeLa and HEK-293T cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1747Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76413 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/20000. Predicted molecular weight: 21 kDa.
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Notes |
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WB
1/1000 - 1/20000. Predicted molecular weight: 21 kDa. |
Target
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Function
General Rab protein regulator required for vesicle formation from the Golgi complex. May control vesicle docking and fusion by mediating the action of Rab GTPases to the SNARE complexes. In addition it inhibits the removal of Rab GTPases from the membrane by GDI. -
Tissue specificity
Ubiquitous. Strongest expression found in placenta, pituitary gland, kidney, lung and stomach. -
Sequence similarities
Belongs to the PRA1 family. -
Developmental stage
In fetal tissues, it is more abundant in kidney and lung. -
Cellular localization
Cell membrane. Cytoplasm. Golgi apparatus. Cytoplasmic vesicle > secretory vesicle > synaptic vesicle. According to some authors, it is an integral membrane protein, while others showed that it is cytoplasmic and membrane-associated to Golgi and synaptic vesicles. - Information by UniProt
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Database links
- Entrez Gene: 10567 Human
- Entrez Gene: 14470 Mouse
- Entrez Gene: 83583 Rat
- Omim: 604925 Human
- SwissProt: Q9UI14 Human
- SwissProt: Q9Z0S9 Mouse
- SwissProt: O35394 Rat
- Unigene: 11417 Human
see all -
Alternative names
- PRA1 domain family 1 antibody
- PRA1 family protein 1 antibody
- PRAF1 antibody
see all
Images
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All lanes : Anti-PRA1 antibody [EPR1747Y] (ab76413) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : RABAC1 knockout HeLa cell lysate
Lane 3 : HEK293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDaLanes 1-3: Merged signal (red and green). Green - ab76413 observed at 21 kDa. Red - loading control ab7291 observed at 50 kDa.
ab76413 Anti-PRA1 antibody [EPR1747Y] was shown to specifically react with PRA1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265702 (knockout cell lysate ab258162) was used. Wild-type and PRA1 knockout samples were subjected to SDS-PAGE. ab76413 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Anti-PRA1 antibody [EPR1747Y] (ab76413) at 1/2000 dilution + 293 cell lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab76413 has been referenced in 1 publication.
- Xiao Y et al. Inhibition of cell proliferation and tumor growth of colorectal cancer by inhibitors of Wnt and Notch signaling pathways. Oncol Lett 12:3695-3700 (2016). WB . PubMed: 27900056