Anti-PRDM4/PFM1 antibody [4B6G1] (ab233759)
Key features and details
- Mouse monoclonal [4B6G1] to PRDM4/PFM1
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-PRDM4/PFM1 antibody [4B6G1]
See all PRDM4/PFM1 primary antibodies -
Description
Mouse monoclonal [4B6G1] to PRDM4/PFM1 -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Orangutan -
Immunogen
Recombinant fragment corresponding to Human PRDM4/PFM1 aa 450-600. Expressed in E.coli.
Database link: Q9UKN5 -
Positive control
- WB: Recombinant human PRDM4/PFM1 (AA: 476-575) protein; PRDM4/PFM1 (AA: 476-575)-hIgGFc transfected HEK-293 whole cell lysate. Flow Cyt: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
4B6G1 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233759 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/2000.
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Flow Cyt |
1/200 - 1/400.
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Notes |
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WB
1/500 - 1/2000. |
Flow Cyt
1/200 - 1/400. |
Target
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Function
May function as a transcription factor involved in cell differentiation. -
Tissue specificity
Expressed in many tissues. Highly expressed in ovary, testis, pancreas, brain, heart and prostate. -
Sequence similarities
Contains 6 C2H2-type zinc fingers.
Contains 1 SET domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 11108 Human
- Entrez Gene: 72843 Mouse
- Entrez Gene: 100173737 Orangutan
- Omim: 605780 Human
- SwissProt: Q9UKN5 Human
- SwissProt: Q80V63 Mouse
- SwissProt: Q5R5M1 Orangutan
- Unigene: 506655 Human
see all -
Alternative names
- MGC45046 antibody
- PFM 1 antibody
- PFM1 antibody
see all
Images
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Anti-PRDM4/PFM1 antibody [4B6G1] (ab233759) at 1/500 dilution + Recombinant human PRDM4/PFM1 (AA: 476-575) protein.
Developed using the ECL technique. -
All lanes : Anti-PRDM4/PFM1 antibody [4B6G1] (ab233759) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : PRDM4/PFM1 (AA: 476-575)-hIgGFc transfected HEK-293T whole cell lysate
Developed using the ECL technique. -
Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling PRDM4/PFM1 with ab233759 at 1/200 dilution (green) compared with a negative control (red).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab233759 has not yet been referenced specifically in any publications.