Overview
- Product nameAnti-PRDM5 antibodySee all PRDM5 primary antibodies ...
- DescriptionRabbit polyclonal to PRDM5
- Tested applicationsWB more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Dog - Immunogen
Synthetic peptide corresponding to a region within N terminal amino acids 36-85 (GPFAGEKRMP EDLDENMDYR LMWEVRGSKG EVLYILDATN PRHSNWLRFV) of Human PRDM5 (NP_061169).
- Positive control721_B cell lysate.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab84572 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 73 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
Target
- FunctionSequence-specific DNA-binding transcription factor. Represses transcription at least in part by recruitment of the histone methyltransferase EHMT2/G9A and histone deacetylases such as HDAC1. Regulates hematopoiesis-associated protein-coding and microRNA (miRNA) genes.
- Sequence similaritiesContains 16 C2H2-type zinc fingers.
Contains 1 SET domain. - Cellular localizationNucleus.
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Database links
- Entrez Gene: 422673 Chicken
- Entrez Gene: 11107 Human
- Entrez Gene: 70779 Mouse
- Entrez Gene: 689788 Rat
- Omim: 614161 Human
- SwissProt: Q0VAI9 Human
- SwissProt: Q0VAJ0 Human
- SwissProt: Q6NXQ7 Human
- SwissProt: Q9NQX1 Human
- SwissProt: Q9CXE0 Mouse
- Unigene: 666782 Human
- Unigene: 669312 Human
- Unigene: 263355 Mouse
see all
Target information above from: UniProt accession
Q9NQX1
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- BCS2 antibodyPFM 2 antibodyPFM2 antibody
- PR domain containing 5 antibodyPR domain containing protein 5 antibodyPR domain zinc finger protein 5 antibodyPR domain-containing protein 5 antibodyPRDM 5 antibodyPRDM5 antibodyPRDM5 protein antibodyPRDM5_HUMAN antibody
see all
Anti-PRDM5 antibody images
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Western blot - PRDM5 antibody (ab84572)Anti-PRDM5 antibody (ab84572) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 73 kDa
Observed band size : 80 kDa (why is the actual band size different from the predicted?)
Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-PRDM5 antibody(ab84572)Immunohistochemistry of Human Small Intestine tissue at an antibody concentration of 5.0µg/ml using anti-PRDM5 antibody (ab84572)
References for Anti-PRDM5 antibody (ab84572)
ab84572 has not yet been referenced specifically in any publications.
