Overview

  • Product nameAnti-PRDM5 antibodySee all PRDM5 primary antibodies ...
  • Description
    Rabbit polyclonal to PRDM5
  • Tested applicationsWB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 36-85 (GPFAGEKRMP EDLDENMDYR LMWEVRGSKG EVLYILDATN PRHSNWLRFV) of Human PRDM5 (NP_061169).

  • Positive control
    • 721_B cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab84572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 73 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.

Target

Anti-PRDM5 antibody images

  • Anti-PRDM5 antibody (ab84572) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 73 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry of Human Small Intestine tissue at an antibody concentration of 5.0µg/ml using anti-PRDM5 antibody (ab84572)

References for Anti-PRDM5 antibody (ab84572)

ab84572 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample Human Cell (Human corneal endothelial cells (HCEC-12))
Specification Human corneal endothelial cells (HCEC-12)
Permeabilization No
Fixative Paraformaldehyde
Username

Mr. Kostadin Rolev

Verified customer

Submitted Apr 29 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Peroxide block (3-4% Hydrogen peroxide) as blocking agent for 5 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA based buffer - Epitope Retreival solution 2 (pH 9) for 20 min Cat No:AR9640
Sample Human Tissue sections (Human corneas)
Specification Human corneas
Permeabilization Yes - Heat mediated at 100oC
Fixative Formaldehyde
Username

Mr. Kostadin Rolev

Verified customer

Submitted Feb 25 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"