Anti-PREB antibody (ab3422)

Overview

  • Product nameAnti-PREB antibodySee all PREB primary antibodies ...
  • Description
    Rabbit polyclonal to PREB
  • SpecificityDetects recombinant mouse PREB (Prolactin Regulatory Element Binding protein).
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Recombinant fragment corresponding to Mouse PREB.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityIgG fraction
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab3422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/500. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).

Target

  • FunctionWas first identified based on its probable role in the regulation of pituitary gene transcription. Binds to the prolactin gene (PRL) promoter and seems to activate transcription (By similarity). Guanine nucleotide exchange factor that activates SARA2. Required for the formation of COPII transport vesicles from the ER.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesContains 3 WD repeats.
  • Cellular localizationNucleus. Endoplasmic reticulum membrane.
  • Target information above from: UniProt accession Q9HCU5 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Mammalian guanine nucleotide exchange factor mSec12 antibody
    • Preb antibody
    • PREB_HUMAN antibody
    • Prolactin regulatory binding element protein antibody
    • Prolactin regulatory element binding antibody
    • Prolactin regulatory element binding protein antibody
    • Prolactin regulatory element-binding protein antibody
    • SEC12 antibody
    see all

Anti-PREB antibody images



  • Predicted band size : 45 kDa

    Western blot detection of recombinant mouse PREB using ab3422.

  • ab3422 staining PREB in mouse skeletal tissue sections by Immunohistochemistry (Frozen sections)

    Tissue was fixed with acetone, blocked with 10% serum for 1 hour at 22°C and permeabilized with 1% Triton X-100. Samples were incubated with primary antibody (1/50 in 10% serum) for 12 hours at 4°C. A Rhodamine red-x conjugated donkey anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

References for Anti-PREB antibody (ab3422)

ab3422 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Skeletal Muscle)
Specification Skeletal Muscle
Fixative Acetone
Permeabilization Yes - 1% Triton-X
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
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Submitted Jun 28 2012

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"