Anti-PREX2 antibody (ab121462)
Key features and details
- Rabbit polyclonal to PREX2
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PREX2 antibody
See all PREX2 primary antibodies -
Description
Rabbit polyclonal to PREX2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen, corresponding to amino acids 686-835 of Human PREX2
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Positive control
- Human stomach tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121462 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment |
Notes |
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IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
Target
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Function
Functions as a RAC1 guanine nucleotide exchange factor (GEF), activating Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol-3,4,5-triphosphate and the beta gamma subunits of heterotrimeric G protein. Mediates the activation of RAC1 in a PI3K-dependent manner. May be an important mediator of Rac signaling, acting directly downstream of both G protein-coupled receptors and phosphoinositide 3-kinase. -
Tissue specificity
Isoform 1 is highly expressed in skeletal muscle, heart and placenta, absent from peripheral blood leukocytes. Isoform 2 is expressed in skeletal muscle, kidney, small intestine, and placenta. Isoform 3 is expressed in the heart. -
Sequence similarities
Contains 2 DEP domains.
Contains 1 DH (DBL-homology) domain.
Contains 2 PDZ (DHR) domains.
Contains 1 PH domain. -
Domain
PH domain confers substrate specificity and recognition. Able to discriminate between RAC1, RHOA, and CDC42.
DH domain alone was unable to confer substrate specificity and recognition. - Information by UniProt
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Database links
- Entrez Gene: 80243 Human
- Omim: 612139 Human
- SwissProt: Q70Z35 Human
- Unigene: 591867 Human
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Alternative names
- 4 antibody
- 5)-dependent Rac exchanger 2 antibody
- 5-trisphosphate-dependent Rac exchanger 2 protein antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab121462 has been referenced in 4 publications.
- Yeung YT et al. CELF2 suppresses non-small cell lung carcinoma growth by inhibiting the PREX2-PTEN interaction. Carcinogenesis N/A:N/A (2019). PubMed: 31241130
- Takeshita S et al. Expression of P-REX2a is associated with poor prognosis in endometrial malignancies. Oncotarget 9:24778-24786 (2018). PubMed: 29872505
- He S et al. Upregulation of PREX2 promotes the proliferation and migration of hepatocellular carcinoma cells via PTEN-AKT signaling. Oncol Lett 11:2223-2228 (2016). Human . PubMed: 26998152
- Liu L et al. Knockdown of PREX2a inhibits the malignant phenotype of glioma cells. Mol Med Rep 13:2301-7 (2016). PubMed: 26795161