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Products:Immunology >> Adaptive Immunity >> T Cells >> Cytotoxic Cells
Overview
Product name
Anti-PRG2 antibody [1.B.787]
See all PRG2 products (4) ...
Description
Mouse monoclonal [1.B.787] to PRG2
Specificity
Specific for eosinophil major basic protein (14kDa) irrespective of the stages of eosinophil activation and can be regarded as a “pan-eosinophil” marker. Recognizes secreted pro-form of MBP (Pregnancy associated MBP, 23.8kDa) and mature form found in the matrix of the eosinophil large specific granule (13.8kDa).
Tested applications
ELISA, IHC-Fr, IHC-P, WBmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse, Rat, Horse
Immunogen
Full length protein (Human).
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 10mM Sodium Azide
Constituents: 1mg/ml BSA, PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
1.B.787
Isotype
IgG1
Research areas
Immunology >> Cell Type Markers >> CD >> NK Cells
Immunology >> Adaptive Immunity >> T Cells >> Cytotoxic Cells
Applications
Show applications key
Our Abpromise guarantee covers the use of ab14462 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
IHC-Fr
IHC-P
WB
Application notes
ELISA: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution. Perform enzymatic antigen retrieval before commencing with IHC staining protocol (0.1% trypsin).
IHC-Fr: Use at an assay dependent dilution. Fix with methanol:acetone.
WB: Use at an assay dependent dilution. Predicted molecular weight: 26.6 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Cytotoxin and helminthotoxin. Also induces non-cytolytic histamine release from human basophils. Involved in antiparasitic defense mechanisms and immune hypersensitivity reactions. The proform acts as a proteinase inhibitor, reducing the activity of PAPPA.
Tissue specificity
High levels of the proform in placenta and pregnancy serum; in placenta, localized to X cells of septa and anchoring villi. Lower levels in a variety of other tissues including kidney, myometrium, endometrium, ovaries, breast, prostate, bone marrow and colon.
Sequence similarities
Contains 1 C-type lectin domain.
Developmental stage
Levels of the proform increase in serum and placenta during pregnancy.
Post-translational
modifications
Nitrated.
Cellular localization
Cytoplasmic vesicle > secretory vesicle. The proform is secreted. The mature protein is found in the matrix of the eosinophil's large specific granule and Secreted. The proform is secreted.
Target information above from: UniProt accessionP13727
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BMPG antibody
- Bone Marrow Proteoglycan antibody
- EMBP antibody
see all
Database links
- Entrez Gene: 5553 Human
- Omim: 605601 Human
- SwissProt: P13727 Human
- Unigene: 512633 Human
References for Anti-PRG2 antibody [1.B.787] (ab14462)
ab14462 has not yet been referenced specifically in any publications.
Publishing research using ab14462? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"