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Product Name
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PRG2 antibody [BMK13]
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See all PRG2 antibodies (7)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [BMK13] to PRG2
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Immunogen
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whole isolated human eosinophils
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Reacts with
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Hu
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Does not react with
(species key)
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Ms, Rat, Hrs
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Specificity
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ab77842 is specific for PRG2 irrespective of the stages of eosinophil activation and can be regarded as a "pan-eosinophil" marker. This antibody will recognize the 23.8 kDa secreted pro-form found in the placenta and serum of pregnant women as well as the 13.8 kDa mature form of the protein which is found in the matrix of the eosinophil large specific granule.
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Tested applications
(see key)
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ELISA, IHC-Fr, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions ELISA: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution. IHC-Fr: Use at an assay dependent dilution. The antibody reacts better in frozen tissues than in paraffin embedded tissues.
WB: Use at an assay dependent dilution. Predicted molecular weight: 25 kDa.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Purified normal human eosinophils.
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Cellular localization
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Secreted. Note=The proform is secreted. The mature protein is found in the matrix of the eosinophil's large specific granule (crystalloid core).
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Research areas
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Immunology >> Cell Type Markers >> CD >> NK Cells Immunology >> Adaptive Immunity >> T Cells >> Cytotoxic Cells
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Relevance
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PRG2 is a cytotoxin and helminthotoxin. It also induces noncytolytic histamine release from human basophils and is involved in antiparasitic defense mechanisms and immune hypersensitivity reactions. The proform acts as a proteinase inhibitor, reducing the activity of PAPPA. In pregnancy serum, the proform exists as a disulfide-linked 2:2 heterotetramer with PAPPA, as a disulfide-linked 2:2 heterotetramer with AGT, and as a complex (probably a 2:2:2 heterohexamer) with AGT and C3dg.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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BMK13
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Isotype
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IgG1
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Purity
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Protein A purified
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Storage buffer
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Preservative: 10mM Sodium Azide Constituents: PBS, 1mg/ml BSA Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.100 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PRG2 antibody [BMK13] is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to PRG2
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PRG2 antibody [BMK13] - more information
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