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Products:Immunology >> Adaptive Immunity >> T Cells >> Cytotoxic Cells
Overview
Product name
Anti-PRG2 antibody [BMK13]
See all PRG2 products (4) ...
Description
Mouse monoclonal [BMK13] to PRG2
Specificity
ab77842 is specific for PRG2 irrespective of the stages of eosinophil activation and can be regarded as a "pan-eosinophil" marker. This antibody will recognize the 23.8 kDa secreted pro-form found in the placenta and serum of pregnant women as well as the 13.8 kDa mature form of the protein which is found in the matrix of the eosinophil large specific granule.
Tested applications
WB, ELISA, IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse, Rat, Horse
Immunogen
whole isolated human eosinophils
Positive control
Purified normal human eosinophils.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 10mM Sodium Azide
Constituents: PBS, 1mg/ml BSA
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
BMK13
Isotype
IgG1
Research areas
Immunology >> Cell Type Markers >> CD >> NK Cells
Immunology >> Adaptive Immunity >> T Cells >> Cytotoxic Cells
Applications
Show applications key
Our Abpromise guarantee covers the use of ab77842 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
ELISA
IHC-P
IHC-Fr
Application notes
ELISA: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
The antibody reacts better in frozen tissues than in paraffin embedded tissues.
WB: Use at an assay dependent dilution. Predicted molecular weight: 25 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Cytotoxin and helminthotoxin. Also induces non-cytolytic histamine release from human basophils. Involved in antiparasitic defense mechanisms and immune hypersensitivity reactions. The proform acts as a proteinase inhibitor, reducing the activity of PAPPA.
Tissue specificity
High levels of the proform in placenta and pregnancy serum; in placenta, localized to X cells of septa and anchoring villi. Lower levels in a variety of other tissues including kidney, myometrium, endometrium, ovaries, breast, prostate, bone marrow and colon.
Sequence similarities
Contains 1 C-type lectin domain.
Developmental stage
Levels of the proform increase in serum and placenta during pregnancy.
Post-translational
modifications
Nitrated.
Cellular localization
Cytoplasmic vesicle > secretory vesicle. The proform is secreted. The mature protein is found in the matrix of the eosinophil's large specific granule and Secreted. The proform is secreted.
Target information above from: UniProt accessionP13727
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BMPG antibody
- Bone marrow proteoglycan antibody
- EMBP antibody
see all
Database links
- Entrez Gene: 5553 Human
- Omim: 605601 Human
- SwissProt: P13727 Human
- Unigene: 512633 Human
References for Anti-PRG2 antibody [BMK13] (ab77842)
ab77842 has not yet been referenced specifically in any publications.
Publishing research using ab77842? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"