PRG2 antibody [BMK13] (ab77842)

Product Name 

PRG2 antibody [BMK13]

Product type 

Primary antibodies

Description 

Mouse monoclonal [BMK13] to PRG2

Immunogen 

whole isolated human eosinophils

Reacts with 

Hu

Does not react with 
(species key)

Ms, Rat, Hrs

Specificity 

ab77842 is specific for PRG2 irrespective of the stages of eosinophil activation and can be regarded as a "pan-eosinophil" marker. This antibody will recognize the 23.8 kDa secreted pro-form found in the placenta and serum of pregnant women as well as the 13.8 kDa mature form of the protein which is found in the matrix of the eosinophil large specific granule.

Tested applications 
(see key)

ELISA, IHC-Fr, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution.

IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
The antibody reacts better in frozen tissues than in paraffin embedded tissues.

WB: Use at an assay dependent dilution. Predicted molecular weight: 25 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Purified normal human eosinophils.

Cellular localization 

Secreted. Note=The proform is secreted. The mature protein is found in the matrix of the eosinophil's large specific granule (crystalloid core).

Research areas 

Immunology >> Cell Type Markers >> CD >> NK Cells
Immunology >> Adaptive Immunity >> T Cells >> Cytotoxic Cells

Relevance 

PRG2 is a cytotoxin and helminthotoxin. It also induces noncytolytic histamine release from human basophils and is involved in antiparasitic defense mechanisms and immune hypersensitivity reactions. The proform acts as a proteinase inhibitor, reducing the activity of PAPPA. In pregnancy serum, the proform exists as a disulfide-linked 2:2 heterotetramer with PAPPA, as a disulfide-linked 2:2 heterotetramer with AGT, and as a complex (probably a 2:2:2 heterohexamer) with AGT and C3dg.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

BMK13

Isotype 

IgG1

Purity 

Protein A purified

Storage buffer 

Preservative: 10mM Sodium Azide
Constituents: PBS, 1mg/ml BSA

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.100 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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