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Anti-PRMT1 antibody [PRMT1-171] (ab12189)

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Overview

Product name

Anti-PRMT1 antibody [PRMT1-171]
See all PRMT1 products (12) ...

Description

Mouse monoclonal [PRMT1-171] to PRMT1

Specificity

This antibody reacts with PRMT1 and does not cross-react with PRMT2, 3, 4, and 5.

Tested applications

ICC/IF, WB, ELISA, IP, Double Immunodiffusionmore details

Cross reactivity

Reacts with

Mouse, Rat, Chicken, Hamster, Cow, Dog, Human, Monkey

Immunogen

Monoclonal Anti-PRMT1 is derived from the PRMT1-171 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with full length human PRMT1.

Positive control

Human kidney cell line 293T.

General notes

If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Monoclonal

Clone number

PRMT1-171

Isotype

IgG1

Research areas

Cancer >> Cell cycle >> Cell cycle inhibitors >> p53 pathway
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Arginine Methylation
Epigenetics and Nuclear Signaling >> Transcription >> Co-factors
Epigenetics and Nuclear Signaling >> Cell cycle >> Cell Cycle Inhibitors >> p53
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> p53

Applications

Show applications key

Our Abpromise guarantee covers the use of ab12189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ICC/IF

     

  • WB

     

  • ELISA

     

  • IP

     

  • Double Immunodiffusion

     

Application notes

DID: Use at an assay dependent dilution.
ELISA: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 2 µg/ml (determined by using a whole cell extract of the human kidney cell line 293T). Predicted molecular weight: 42 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway.

Tissue specificity

Widely expressed.

Sequence similarities

Belongs to the protein arginine N-methyltransferase family.

Cellular localization

Nucleus. Cytoplasm > cytosol.

Target information above from: UniProt accessionQ99873 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • ANM 1 antibody
  • ANM1 antibody
  • ANM1_HUMAN antibody
  • complete cds antibody
  • HCP 1 antibody
  • HCP1 antibody
  • Heterogeneous nuclear ribonucleoprotein methyltransferase 1 like 2 antibody
  • Heterogeneous nuclear ribonucleoproteins methyltransferase like 2 antibody
  • Heterogeneous nuclear ribonucleoproteins methyltransferase like2 antibody
  • Histone-arginine N-methyltransferase PRMT1 antibody
  • HMT 2 antibody
  • HMT1 (hnRNP methyltransferase antibody
  • HMT1 (hnRNP methyltransferase S. cerevisiae) like 2 antibody
  • HMT1 (hnRNP methyltransferase S. cerevisiae)-like 2 antibody
  • HMT1 hnRNP methyltransferase antibody
  • HMT1 hnRNP methyltransferase like 2 (S. cerevisiae) antibody
  • HMT1 hnRNP methyltransferase like 2 antibody
  • HMT1 hnRNP methyltransferase-like 2 (S. cerevisiae) antibody
  • HMT2 antibody
  • HRMT1 L2 antibody
  • HRMT1L 2 antibody
  • HRMT1L2 antibody
  • Human mRNA for suppressor for yeast mutant antibody
  • Human mRNA for suppressor for yeast mutant complete cds antibody
  • Interferon receptor 1 bound protein 4 antibody
  • Interferon receptor 1 bound protein4 antibody
  • Interferon receptor 1-bound protein 4 antibody
  • Interferon receptor 1bound protein 4 antibody
  • IR1 B4 antibody
  • IR1B 4 antibody
  • IR1B4 antibody
  • Mrmt 1 antibody
  • Mrmt1 antibody
  • PRMT 1 antibody
  • PRMT1 antibody
  • Protein arginine methyltransferase 1 antibody
  • Protein arginine N methyltransferase 1 antibody
  • Protein arginine N methyltransferase1 antibody
  • Protein arginine N-methyltransferase 1 (EC 2.1.1.-) (Interferon receptor 1-bound protein 4). antibody
  • Protein arginine N-methyltransferase 1 antibody
  • R1B4 antibody
  • S. cerevisiae like 2 antibody
  • S. cerevisiae)-like 2 antibody
see all

Database links

see all

References for Anti-PRMT1 antibody [PRMT1-171] (ab12189)

This product has been referenced in:

  • Butler JS  et al. Protein-arginine Methyltransferase 1 (PRMT1) Methylates Ash2L, a Shared Component of Mammalian Histone H3K4 Methyltransferase Complexes. J Biol Chem 286:12234-44 (2011). ICC/IF; Human.Read more (PubMed: 21285357) »
  • Infantino S  et al. Arginine methylation of the B cell antigen receptor promotes differentiation. J Exp Med 207:711-9 (2010). Mouse.Read more (PubMed: 20231378) »

See all 3 publications for this product

Publishing research using ab12189? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"