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- Secondary antibodies
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Product name
Anti-PRMT1 antibody
See all PRMT1 products (12) ...
Description
Rabbit polyclonal to PRMT1
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide conjugated to KLH, corresponding a regio of amino acids 1-100 of Human PRMT 1.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS with 0.1% sodium azide
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cell cycle >> Cell cycle inhibitors >> p53 pathway
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Arginine Methylation
Epigenetics and Nuclear Signaling >> Transcription >> Co-factors
Epigenetics and Nuclear Signaling >> Cell cycle >> Cell Cycle Inhibitors >> p53
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> p53
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Western blot - PRMT1 antibody (ab3769)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3769 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/100 - 1/500. Predicted molecular weight: 40 kDa.
ELISA
ELISA: 1/1000.ELISA: 1/1000.
Target
Function
Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway.
Tissue specificity
Widely expressed.
Sequence similarities
Belongs to the protein arginine N-methyltransferase family.
Cellular localization
Nucleus. Cytoplasm > cytosol.
Target information above from: UniProt accessionQ99873
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- complete cds antibody
- S. cerevisiae)-like 2 antibody
- ANM 1 antibody
see all
Database links
- Entrez Gene: 3276 Human
- Omim: 602950 Human
- SwissProt: Q99873 Human
- Unigene: 20521 Human
Anti-PRMT1 antibody images:
Western blot - PRMT1 antibody (ab3769)
Predicted band size : 40 kDa
Western blot analysis in whole HL60 cell lysate: PRMT1 (Arrow) was detected using ab3769. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
References for Anti-PRMT1 antibody (ab3769)
ab3769 has not yet been referenced specifically in any publications.
Publishing research using ab3769? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"