Overview

  • Product nameAnti-PRMT1 antibodySee all PRMT1 primary antibodies ...
  • Description
    Rabbit polyclonal to PRMT1
  • Tested applicationsICC/IF, IHC-P, WB, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human
    Predicted to work with: Cow, Non Human Primates
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human PRMT1.

    (Peptide available as ab73687.)

  • Positive control
    • This antibody gave a positive signal in WB against Caco 2 Whole Cell, SW480 Whole Cell and Mouse Thymus Tissue. In IF against HeLa Cells and IHC against Human Hippocampus

Properties

Applications

Our Abpromise guarantee covers the use of ab73246 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42, 39 kDa (predicted molecular weight: 42 kDa).Can be blocked with Human PRMT1 peptide (ab73687).
IHC-Fr Use at an assay dependent concentration. PubMed: 24663083
IP Use at an assay dependent concentration.

Target

  • FunctionArginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the protein arginine N-methyltransferase family.
  • Cellular localizationNucleus. Cytoplasm > cytosol.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANM 1 antibody
    • ANM1 antibody
    • ANM1_HUMAN antibody
    • complete cds antibody
    • HCP 1 antibody
    • HCP1 antibody
    • Heterogeneous nuclear ribonucleoprotein methyltransferase 1 like 2 antibody
    • Heterogeneous nuclear ribonucleoproteins methyltransferase like 2 antibody
    • Heterogeneous nuclear ribonucleoproteins methyltransferase like2 antibody
    • Histone-arginine N-methyltransferase PRMT1 antibody
    • HMT 2 antibody
    • HMT1 (hnRNP methyltransferase antibody
    • HMT1 (hnRNP methyltransferase S. cerevisiae) like 2 antibody
    • HMT1 hnRNP methyltransferase antibody
    • HMT1 hnRNP methyltransferase like 2 (S. cerevisiae) antibody
    • HMT1 hnRNP methyltransferase like 2 antibody
    • HMT1 hnRNP methyltransferase-like 2 (S. cerevisiae) antibody
    • HMT2 antibody
    • HRMT1 L2 antibody
    • HRMT1L 2 antibody
    • HRMT1L2 antibody
    • Human mRNA for suppressor for yeast mutant antibody
    • Human mRNA for suppressor for yeast mutant complete cds antibody
    • Interferon receptor 1 bound protein 4 antibody
    • Interferon receptor 1 bound protein4 antibody
    • Interferon receptor 1-bound protein 4 antibody
    • Interferon receptor 1bound protein 4 antibody
    • IR1 B4 antibody
    • IR1B 4 antibody
    • IR1B4 antibody
    • Mrmt 1 antibody
    • Mrmt1 antibody
    • PRMT 1 antibody
    • PRMT1 antibody
    • Protein arginine methyltransferase 1 antibody
    • Protein arginine N methyltransferase 1 antibody
    • Protein arginine N methyltransferase1 antibody
    • Protein arginine N-methyltransferase 1 antibody
    • R1B4 antibody
    • S. cerevisiae like 2 antibody
    see all

Anti-PRMT1 antibody images

  • All lanes : Anti-PRMT1 antibody (ab73246) at 1 µg/ml

    Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate (ab76828)
    Lane 2 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate (ab76999)
    Lane 3 : Thymus (Mouse) Tissue Lysate (ab76823)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 25 kDa (possible non-specific binding),38 kDa (possible isoform),90 kDa (possible non-specific binding).

    Exposure time : 2 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 1% milk before being incubated with ab73246 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

     

    This antibody was raised against an immunogen that is predicted to recognize isoforms 1,2,3 and 4 of human PRMT1. The predicted molecular weights of isoforms 1,2,3 and 4 are 41kDa, 39kDa, 39kDa and 40kDa respectively.

  • PRMT1 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Rabbit polyclonal to PRMT1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab73246.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 42kDa: PRMT1; non specific - 30kDa: We are unsure as to the identity of this extra band.
  • ICC/IF image of ab73246 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73246, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2 cells at 1µg/ml.
  • IHC image of PRMT1 staining in Human Hippocampus FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab73246, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

References for Anti-PRMT1 antibody (ab73246)

This product has been referenced in:
  • Jung CS  et al. Endogenous nitric-oxide synthase inhibitor ADMA after acute brain injury. Int J Mol Sci 15:4088-103 (2014). IHC-Fr ; Rat . Read more (PubMed: 24663083) »
  • Liu H  et al. Variance of DDAH/PRMT/ADMA pathway in atrial fibrillation dogs. Biochem Biophys Res Commun 377:884-8 (2008). WB ; Dog . Read more (PubMed: 18951871) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (primary human cells skin)
Loading amount 20 µg
Specification primary human cells skin
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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Submitted Jun 24 2011

Application Western blot
Sample Human Cell lysate - whole cell (u2os)
Loading amount 80 µg
Specification u2os
Gel Running Conditions Non-reduced Denaturing (4-20)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jun 08 2011

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cultured Cells (u2os)
Specification u2os
Fixative Formaldehyde
Permeabilization Yes - triton
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Dec 14 2010

Application Western blot
Sample Rat Cell lysate - whole cell (PC12, RIE, 9L, Rt Thymus)
Loading amount 20 µg
Specification PC12, RIE, 9L, Rt Thymus
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Jan 11 2010

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