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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human PRMT1.
(Peptide available as ab73687.)
Our Abpromise guarantee covers the use of ab73246 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-P||Use a concentration of 5 µg/ml.|
|IHC||Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 42, 39 kDa (predicted molecular weight: 42 kDa).Can be blocked with Human PRMT1 peptide (ab73687).|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 24663083|
|IP||Use at an assay dependent concentration.|
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 1% milk before being incubated with ab73246 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
This antibody was raised against an immunogen that is predicted to recognize isoforms 1,2,3 and 4 of human PRMT1. The predicted molecular weights of isoforms 1,2,3 and 4 are 41kDa, 39kDa, 39kDa and 40kDa respectively.
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