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- Secondary antibodies
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Product name
Anti-PRMT4 antibody
See all PRMT4 products (8) ...
Description
Rabbit polyclonal to PRMT4
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthetic peptides, corresponding to amino acids 45-69 (NGEIQRHAEQQALRLEVRAGPDAAG) and 595-608 (SPMSIPTNTMHYGS) of Human PRMT4.
Positive control
Tested with HeLa cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS, 0.1mg/ml BSA
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Cofactors, Vitamins / minerals >> Co-factors
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Lipid and lipoprotein metabolism >> Lipid metabolism
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> p53 pathway
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Arginine Methylation
Epigenetics and Nuclear Signaling >> Transcription >> Co-factors
Epigenetics and Nuclear Signaling >> Cell cycle >> Cell Cycle Inhibitors >> p53
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Co-activators/co-repressors
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> p53
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Western blot - PRMT4 antibody (ab13707)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab13707 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 60 kDa.
Target
Function
Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs.
Tissue specificity
Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.
Sequence similarities
Belongs to the protein arginine N-methyltransferase family.
Post-translational
modifications
Auto-methylated on Arg-550. Methylation enhances transcription coactivator activity. Methylation is required for its role in the regulation of pre-mRNA alternative splicing.
Phosphorylation at Ser-216 interferes with S-adenosyl-L-methionine binding and strongly reduces methyltransferase activity (By similarity). Phosphorylation at Ser-216 is strongly increased during mitosis, and decreases rapidly to a very low, basal level after entry into the G1 phase of the cell cycle. Phosphorylation at Ser-216 may promote location in the cytosol.
Cellular localization
Nucleus. Cytoplasm. Mainly nuclear during the G1, S and G2 phases of the cell cycle. Cytoplasmic during mitosis, after breakup of the nuclear membrane.
Target information above from: UniProt accessionQ86X55
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CARM1 antibody
- CARM1_HUMAN antibody
- Coactivator associated arginine methyltransferase 1 antibody
see all
Database links
- Entrez Gene: 10498 Human
- Entrez Gene: 59035 Mouse
- Omim: 603934 Human
- SwissProt: Q86X55 Human
- SwissProt: Q9WVG6 Mouse
- Unigene: 323213 Human
- Unigene: 178115 Mouse
Anti-PRMT4 antibody images:
Western blot - PRMT4 antibody (ab13707)
Western blot analysis of PRMT4 in cell lysates from HeLa cells using ab13707 at 0.5 mg/ml. Twenty microgram of HeLa cell lysate was loaded per well of mini gel.
References for Anti-PRMT4 antibody (ab13707)
ab13707 has not yet been referenced specifically in any publications.
Publishing research using ab13707? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"