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Anti-PRMT5 antibody [PRMT5-21] (ab12191)

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Overview

Product name

Anti-PRMT5 antibody [PRMT5-21]
See all PRMT5 products (8) ...

Description

Mouse monoclonal [PRMT5-21] to PRMT5

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Mouse, Rat, Chicken, Hamster, Cow, Dog, Human, Monkey

Immunogen

Antibody was produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with PRMT5 containing the C-terminal region of the Human PRMT5 (amino acids 315-637).

Epitope

Monoclonal Anti-PRMT5 (protein-arginine methyl transferase 5) recognizes an epitope within the C-terminal region of PRMT5.

Positive control

Whole cell extract of human melanoma cell line G361.

General notes

If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Monoclonal

Clone number

PRMT5-21

Isotype

IgG2a

Research areas

Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Arginine Methylation
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation

Applications

Show applications key

Our Abpromise guarantee covers the use of ab12191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • WB

     

  • ELISA

     

Application notes

ELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 2 µg/ml (determined by using a whole cell extract of human melanoma cell line G361). Predicted molecular weight: 70 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H. Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SUPT5H transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Methylates histone H2A and H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription. Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. Methylates RPS10.

Tissue specificity

Ubiquitous.

Sequence similarities

Belongs to the protein arginine N-methyltransferase family.

Post-translational
modifications

Disulfide bonds and non-covalent association mediate homooligomers formation.

Cellular localization

Cytoplasm. Nucleus.

Target information above from: UniProt accessionO14744 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • 72 kDa ICln binding protein antibody
  • 72 kDa ICln-binding protein antibody
  • ANM5_HUMAN antibody
  • Histone-arginine N-methyltransferase PRMT5 antibody
  • HMT1 hnRNP methyltransferase like 5 antibody
  • HOMOLOG OF; SKB1 antibody
  • HRMT1L5 antibody
  • IBP72 antibody
  • Jak-binding protein 1 antibody
  • JBP 1 antibody
  • JBP1 antibody
  • PRMT 5 antibody
  • PRMT5 antibody
  • Protein arginine methyltransferase 5 antibody
  • Protein arginine N methyltransferase 5 antibody
  • Protein arginine N-methyltransferase 5 antibody
  • S. POMBE antibody
  • S. POMBE antibody
  • S. POMBE HOMOLOG OF; SKB1 antibody
  • SHK1 KINASE BINDING PROTEIN 1 antibody
  • Shk1 kinase binding protein 1 homolog antibody
  • SHK1 KINASE-BINDING PROTEIN 1 antibody
  • Shk1 kinase-binding protein 1 homolog antibody
  • SKB 1 antibody
  • SKB1 antibody
  • SKB1 homolog antibody
  • SKB1: SKB1 homolog (S. pombe) antibody
  • SKB1Hs antibody
see all

Database links

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References for Anti-PRMT5 antibody [PRMT5-21] (ab12191)

This product has been referenced in:

  • van Bergeijk J  et al. The spinal muscular atrophy gene product regulates neurite outgrowth: importance of the C terminus. FASEB J 21:1492-502 (2007).Read more (PubMed: 17317728) »

See 1 publication for this product

Publishing research using ab12191? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"