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ab30553
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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab31751? Please let us know so that we can cite the reference in this datasheet
ab31751 has been referenced in 1 publications.
- Vagin VV et al. Proteomic analysis of murine Piwi proteins reveals a role for arginine methylation in specifying interaction with Tudor family members. Genes Dev 23:1749-62 (2009). ICC/IF; Mouse. PubMed: 19584108
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PRMT5 antibody (ab31751)
Image courtesy of Human Protein Atlas
ab31751 staining PRMT5 in human prostate, showing a distinct and strong staining pattern in glandular cells. Paraffin embedded human skin tissue was incubated with ab31751 (1/150 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab31751 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
Western blot - PRMT5 antibody (ab31751)
All lanes : Anti-PRMT5 antibody (ab31751) at 1 µg/ml
Lane 1 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 :
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 72 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Additional bands at : 110 kDa,37 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 6 minutes
PRMT5 antibody for WB in Human (31751)
PRMT5 antibody for Western blot in Human (31751)
PRMT5 antibody for Immunocytochemistry/ Immunofluorescence in Rabbit (31751)
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