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Overview
Product name
Anti-PRMT5 antibody
See all PRMT5 products (8) ...
Description
Rabbit polyclonal to PRMT5
Tested applications
ICC/IF, WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Xenopus laevis, Zebrafish
Immunogen
Synthetic peptide derived from within residues 600 to the C-terminus of Human PRMT5.
(Peptide available as ab30553.)
Positive control
HL60 (Human acute promyelocytic leukemia cell line) Whole Cell Lysate; HEK 293 (Human embryonic kidney cell line) Whole Cell Lysate; NIH 3T3 (Mouse embryonic fibroblast cell line) Whole cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab31751 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF
Three stars (1 Abreview)- 1 Image
IHC-P
Application notes
ICC/IF: Use at an assay dependent dilution (PMID 19584108).
IHC-P: 1/150. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa). ab31751 shows weak staining in mouse samples.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H. Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SUPT5H transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Methylates histone H2A and H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription. Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. Methylates RPS10.
Tissue specificity
Ubiquitous.
Sequence similarities
Belongs to the protein arginine N-methyltransferase family.
Post-translational
modifications
Disulfide bonds and non-covalent association mediate homooligomers formation.
Cellular localization
Cytoplasm. Nucleus.
Target information above from: UniProt accessionO14744
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 72 kDa ICln binding protein antibody
- 72 kDa ICln-binding protein antibody
- ANM5_HUMAN antibody
see all
Database links
- Entrez Gene: 10419 Human
- Entrez Gene: 27374 Mouse
- Entrez Gene: 368664 Zebrafish
- Omim: 604045 Human
- SwissProt: O14744 Human
- SwissProt: Q8CIG8 Mouse
- Unigene: 367854 Human
- Unigene: 196585 Mouse
Anti-PRMT5 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PRMT5 antibody (ab31751)
Image courtesy of Human Protein Atlas
ab31751 staining PRMT5 in human prostate, showing a distinct and strong staining pattern in glandular cells. Paraffin embedded human skin tissue was incubated with ab31751 (1/150 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab31751 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
Western blot - PRMT5 antibody (ab31751)
All lanes : Anti-PRMT5 antibody (ab31751) at 1 µg/ml
Lane 1 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 72 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Additional bands at : 110 kDa,37 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 6 minutes
References for Anti-PRMT5 antibody (ab31751)
This product has been referenced in:
- Vagin VVet al. Proteomic analysis of murine Piwi proteins reveals a role for arginine methylation in specifying interaction with Tudor family members. Genes Dev 23:1749-62 (2009). ICC/IF; Mouse.Read more (PubMed: 19584108) »
See 1 publication for this product
Publishing research using ab31751? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"